Li Linghua, Hu Fengyu, Chen Wanshan, Tang Xiaoping, Song Weinan, Kuang Yangling, Cai Weiping, Chen Xiejie
Department of Infectious Diseases, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
Scand J Infect Dis. 2011 Aug;43(8):616-24. doi: 10.3109/00365548.2011.559649. Epub 2011 Feb 18.
Penicillium marneffei is an opportunistic fungus that may cause fatal disease, and usually infects acquired immune deficiency syndrome (AIDS) patients. The molecular epidemiology of this fungus remains enigmatic.
A multilocus microsatellite typing (MLMT) system based on 11 microsatellite loci was applied to 169 unrelated isolates of P. marneffei obtained from AIDS patients, in order to identify their genetic diversity. These patients came from the provinces of Guangdong and Guangxi, areas endemic for P. marneffei in China.
For the overall population, the average number of alleles per locus ranged from 3 to 8 (mean 5.5), while the discriminatory power (DP) of each locus ranged from 0.235 to 0.651 (mean 0.512). By combining the information generated for 11 loci, MLMT detected 159 different multilocus genotypes (MTs), resulting in a high degree of discrimination (DP = 0.999). One hundred and sixty-nine isolates were further clustered into 9 types (from A to I) at the similarity coefficient of 0.80, with type A (80 isolates) and type B (60 isolates) being the most common types. Within 5 subpopulations from different regions of China, the distribution of MTs of P. marneffei isolates was diverse. Although 169 isolates shared a high genetic similarity (range 0.71-0.933), isolates from Guangxi and Guangdong provinces could be differentiated from each other and clustered into 2 categories by unweighted pair-group method with arithmetic mean (UPGMA) cluster analysis.
By MLMT, the genetic diversity of clinical P. marneffei isolates could be discriminated, the dominant strain of P. marneffei cultured from AIDS patients in China could be identified, and clinical isolates of P. marneffei from Guangxi Province could be differentiated from those from Guangdong Province.
马尔尼菲青霉菌是一种可导致致命疾病的机会性真菌，通常感染获得性免疫缺陷综合征（AIDS）患者。这种真菌的分子流行病学仍然不明。
基于11个微卫星位点的多位点微卫星分型（MLMT）系统应用于从AIDS患者中获得的169株无关的马尔尼菲青霉菌分离株，以确定其遗传多样性。这些患者来自中国马尔尼菲青霉菌的地方性流行地区广东省和广西壮族自治区。
对于总体人群，每个位点的等位基因平均数为3至8（平均5.5），而每个位点的鉴别力（DP）为0.235至0.651（平均0.512）。通过合并11个位点产生的信息，MLMT检测到159种不同的多位点基因型（MTs），鉴别度很高（DP = 0.999）。在相似系数为0.80时，169株分离株进一步聚类为9种类型（从A到I），其中A类型（80株）和B类型（60株）是最常见的类型。在中国不同地区的5个亚群中，马尔尼菲青霉菌分离株的MTs分布多样。尽管169株分离株具有较高的遗传相似性（范围为0.71 - 0.933），但通过非加权组平均法（UPGMA）聚类分析，来自广西和广东的分离株可以相互区分并聚类为2类。
通过MLMT，可以鉴别临床马尔尼菲青霉菌分离株的遗传多样性，确定中国AIDS患者培养的马尔尼菲青霉菌的优势菌株，并且可以区分来自广西和广东的马尔尼菲青霉菌临床分离株。
