Hwang Jae Woong, Choi Young Bum, Park Sunghoon, Choi Cha Yong, Lee Eun Yeol
School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea.
Biodegradation. 2007 Feb;18(1):91-101. doi: 10.1007/s10532-006-9040-3. Epub 2006 Feb 9.
A two-stage reactor system was developed for the continuous degradation of gas-phase trichloroethylene (TCE). Methylosinus trichosporium OB3b was immobilized on activated carbon in a TCE degradation reactor, trickling biofilter (TBF). The TBF was coupled with a continuous stirred tank reactor (CSTR) to allow recirculation of microbial cells from/to the TBF for the reactivation of inactivated cells during TCE degradation. The mass transfer aspect of the TBF was analyzed, and mass transfer coefficient of 3.9 h(-1) was estimated. The loss of soluble methane monooxygenase (sMMO) activity was modeled based on a material balance on the CSTR and TBF, and transformation capacity (T (c)) was determined to be 20.2 micromol mg(-1). Maximum TCE degradation rate of 525 mg 1(-1) d(-1) was obtained and reactor has been stably operated for more than 270 days.
开发了一种两级反应器系统用于气相三氯乙烯（TCE）的连续降解。将甲基孢囊菌OB3b固定在TCE降解反应器（滴滤生物滤池，TBF）中的活性炭上。TBF与连续搅拌釜式反应器（CSTR）相连，以便在TCE降解过程中使微生物细胞在TBF之间循环，从而使失活细胞重新活化。分析了TBF的传质情况，估计传质系数为3.9 h(-1)。基于CSTR和TBF的物料平衡对可溶性甲烷单加氧酶（sMMO）活性的损失进行了建模，确定转化能力（T(c)）为20.2 μmol mg(-1)。获得了525 mg l(-1) d(-1)的最大TCE降解率，并且该反应器已稳定运行超过270天。
