Bistey Tamás, Jenei Attila, Szondy Zsuzsa, Nagy P István, Hegedus Csaba
Debreceni Egyetem, Orvos- és Egészségtudományi Centrum, Fogorvostudományi Kar, Fogpótlástani Tanszék.
Fogorv Sz. 2005 Dec;98(6):245-50.
The aim of the present study was to investigate the effects of hydrogen peroxide solutions (10-30%) on the enamel surface and the inner structure of human teeth. Prepared enamel samples were exposed to various concentrations of hydrogen peroxide solutions for one hour. Changes in the enamel were analyzed by two different methods. The inner structure was studied by the Ellman's reagent to detect possible changes in the amount of free thiol groups in the remaining organic phase of matured enamel. Surface alterations were imaged by atomic force microscope. Ellman's reaction revealed the presence of three groups of free thiols: one with easy, the second one with medium and the third one with difficult accessibility by hydrogen peroxide. Atomic force microscopy revealed severe alterations on the enamel surface after treatment with both low and high concentration of hydrogen peroxide. Our observations indicated that hydrogen peroxide induced alterations in the surface structure and in the inner phases of mature enamel in both low and high concentrations.
本研究的目的是调查过氧化氢溶液（10%-30%）对人牙齿釉质表面和内部结构的影响。制备好的釉质样本暴露于不同浓度的过氧化氢溶液中一小时。通过两种不同方法分析釉质的变化。利用埃尔曼试剂研究内部结构，以检测成熟釉质剩余有机相中游离巯基数量的可能变化。通过原子力显微镜对表面变化进行成像。埃尔曼反应揭示了三组游离巯基的存在：一组易于被过氧化氢接触，第二组中等程度可及，第三组难以被过氧化氢接触。原子力显微镜显示，用低浓度和高浓度过氧化氢处理后，釉质表面出现严重变化。我们的观察表明，低浓度和高浓度的过氧化氢都会导致成熟釉质的表面结构和内部相发生变化。
