Nascimento J P, Hallam N F, Mori J, Field A M, Clewley J P, Brown K E, Cohen B J
Virus Reference Laboratory, Central Public Health Laboratory, London, United Kingdom.
J Med Virol. 1991 Feb;33(2):77-82. doi: 10.1002/jmv.1890330203.
Evidence of B19 parvovirus infection was sought by in situ hybridisation with biotinylated probes in 65 tissue samples from 32 pregnancies (fetuses, products of conception and/or placentas). Twenty-seven samples were reactive and the results were confirmed by other methods for B19 virus detection in 22 cases. The other methods used were in situ hybridisation with 3H and 35S labelled probes; dot-blot hybridisation with biotin and 32P labelled probes; polymerase chain reaction assay; negative stain and thin section electron microscopy; and radioimmunoassay for B19 antigen. The five false positive results by in situ hybridisation with biotinylated probes were considered to be due to non-specific biotin capture and were more frequent with unfixed samples than with formalin fixed material. It was concluded that while biotinylated probes offered advantages over radioactive probes for detecting B19 DNA by in situ hybridisation, positive findings should be confirmed by other methods.
采用生物素化探针原位杂交法，对32例妊娠组织样本（胎儿、妊娠产物和/或胎盘）共65份进行检测，以寻找B19细小病毒感染的证据。27份样本呈阳性反应，其中22例通过其他检测B19病毒的方法得到了验证。所采用的其他方法包括：用3H和35S标记探针的原位杂交法；用生物素和32P标记探针的斑点杂交法；聚合酶链反应分析；负染色和超薄切片电子显微镜检查；以及B19抗原放射免疫测定法。生物素化探针原位杂交法出现的5例假阳性结果被认为是由于非特异性生物素捕获所致，与福尔马林固定材料相比，未固定样本中出现假阳性的频率更高。得出的结论是，虽然生物素化探针在原位杂交检测B19 DNA方面比放射性探针具有优势，但阳性结果应用其他方法加以验证。
