Lasham A, Vreugdenhil E, Bateson A N, Barnard E A, Darlison M G
MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, England.
J Neurochem. 1991 Jul;57(1):352-5. doi: 10.1111/j.1471-4159.1991.tb02135.x.
A series of genomic clones containing DNA that encodes the chicken gamma-aminobutyric acidA (GABAA) receptor beta 4 subunit have been isolated. These have been restriction mapped and partially sequenced to determine the structural organization and the size of the beta 4-subunit gene. This gene, which comprises nine exons, spans more than 65 kb. The organization of the chicken GABAA receptor beta 4-subunit gene has been compared to that of the murine GABAA receptor delta-subunit gene and to those of the genes that encode other members of the ligand-gated ion-channel superfamily, namely muscle and neuronal nicotinic acetylcholine receptors (AChRs). Although the positions of the intron/exon boundaries of GABAA receptor subunit genes are seen to be highly conserved, there are significant differences between the genes that encode GABAA receptor and AChR subunits. These results are discussed in relation to the proposal that this superfamily of ligand-gated ion-channel receptor genes arose by duplication of an ancestral receptor gene.
一系列包含编码鸡γ-氨基丁酸A（GABAA）受体β4亚基DNA的基因组克隆已被分离出来。这些克隆已进行限制性图谱分析并部分测序，以确定β4亚基基因的结构组织和大小。该基因由九个外显子组成，跨度超过65kb。已将鸡GABAA受体β4亚基基因的组织与小鼠GABAA受体δ亚基基因以及编码配体门控离子通道超家族其他成员（即肌肉和神经元烟碱型乙酰胆碱受体（AChRs））的基因的组织进行了比较。尽管GABAA受体亚基基因的内含子/外显子边界位置被认为高度保守，但编码GABAA受体和AChR亚基的基因之间存在显著差异。结合配体门控离子通道受体基因这个超家族是由一个祖先受体基因复制产生的这一观点，对这些结果进行了讨论。
