[重组人生长激素对大鼠脓毒症肠黏膜屏障保护作用的可能机制研究]

[Studies of the possible mechanisms for protective effects of recombinant human growth hormone on intestinal mucosa barrier in rat sepsis].

作者信息

Huang Ying, Yi Cheng, Long Yang, Zhao Qiu-ling, Jiang Cong-xun, Cui Yong-xia, He Rong-hua, Wang Shu-ren

机构信息

Department of Pathophysiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Jan;37(1):10-3.

PMID:16468631

Abstract

OBJECTIVE

To investigate the protective effects of recombinant human growth hormone (rhGH) on intestinal mucosal barrier in rat sepsis and explore its possible mechanisms.

METHODS

E. coli was injected intraperitoneally to produce rats sepsis models. Forty-two female SD rats were randomly divided into the control group (group C), sepsis group (group S) and treatment group (group T). Group S and group T were further divided into 1 d and 3 d subgroups (T1d,T3d, Sld, S3d), respectively. The expression of IGF-1 mRNA in liver, expression of Bcl-2 protein in intestine, bacteria translocation, the levels of growth hormone(GH) and insulin-like growth factor-1 (IGF-1) in plasma, and the histological appearance of intestine were determined dynamically by means of RT-PCR, radioimmunoassay, immunohistochemical staining and other corresponding methods, respectively.

RESULTS

(1) rhGH could significantly attenuate intestinal mucosal injuries and ameliorate bacteria translocation on sepsis rats. (2) The levels of Bcl-2 protein expression in intestine in group T (T1d:2441 +/- 117; T3d: 3628 +/- 235) were obviously higher than those of group S (S1d: 321 +/- 36; S3d: 1873 +/- 57) (P < 0.01). (3) The plasma levels of GH in group T (T1d: 1.28 +/- 0.24 microg/L; T3d: 2.14 +/- 0.48 microg/L) increased markedly than those of group S (S1d: 0.74 -/+ 0.12 microg/L; S3d: 0.60 +/- 0.18 microg/L) (P < 0.01). (4) The plasma levels of IGF-1 in group T (Tld: 168.94 +/- 65.67 microg/L; T3d: 201.56 +/- 64.98 microg/L) elevated significantly than those of group S (Sld: 116.72 +/- 13.96 microg/L; S3d:107.50 +/- 23.53 microg/L) (P < 0.05). (5) The levels of liver IGF-1 mRNA in group T (Tld: 0.98 +/- 0.20; T3d: 1.76 +/- 0.17) were significantly higher than those in group S (S1d: 0.38 +/- 0.09; S3d: 0.46 +/- 0.10) (P < 0.01).

CONCLUSION

rhGH conferred protective efficacy in maintaining the integrity of intestinal mucosal barrier against sepsis in rat. The possible mechanisms might involve the rhGH-diminished apoptosis of intestinal mucosa cells and the rhGH-maintained intestinal mucosa barrier via the roles of GH and IGF-1.

摘要

目的

探讨重组人生长激素（rhGH）对大鼠脓毒症肠黏膜屏障的保护作用及其可能机制。

方法

腹腔注射大肠杆菌制备大鼠脓毒症模型。42只雌性SD大鼠随机分为对照组（C组）、脓毒症组（S组）和治疗组（T组）。S组和T组再分别分为1天和3天亚组（T1d、T3d、S1d、S3d）。分别采用RT-PCR、放射免疫分析、免疫组织化学染色等相应方法动态检测肝脏中IGF-1 mRNA的表达、肠组织中Bcl-2蛋白的表达、细菌移位、血浆中生长激素（GH）和胰岛素样生长因子-1（IGF-1）的水平以及肠组织学形态。

结果

（1）rhGH能显著减轻脓毒症大鼠肠黏膜损伤，改善细菌移位。（2）T组肠组织中Bcl-2蛋白表达水平（T1d：2441±117；T3d：3628±235）明显高于S组（S1d：321±36；S3d：1873±57）（P<0.01）。（3）T组血浆GH水平（T1d：1.28±0.24μg/L；T3d：2.14±0.48μg/L）显著高于S组（S1d：0.74±0.12μg/L；S3d：0.60±0.18μg/L）（P<0.01）。（4）T组血浆IGF-1水平（T1d：168.94±65.67μg/L；T3d：201.56±64.98μg/L）明显高于S组（S1d：116.72±13.96μg/L；S3d：107.50±23.53μg/L）（P<0.05）。（5）T组肝脏IGF-1 mRNA水平（T1d：0.98±0.20；T3d：1.76±0.17）显著高于S组（S1d：0.38±0.09；S3d：0.46±0.10）（P<0.01）。