[全氟碳吸入雾化对急性肺损伤猪肠黏膜细胞凋亡的影响]

[Effect of vaporized perfluorocarbon inspiration on cell apoptosis of intestine mucosa: experiment with pigs with acute lung injury].

作者信息

Qin Xin, Liu You-ning

机构信息

Department of Respiratory Medicine, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2007 Feb 13;87(7):493-6.

PMID:17459232

Abstract

OBJECTIVE

To investigate the effect of vaporized perfluorocarbon (PFC) inspiration on cell apoptosis of intestine mucosa.

METHODS

18 piglets are randomly assigned to 3 groups, VMV group (n=8), undergoing intra-bronchial infusion of dioctyl sodium sulfosuccinate so as to cause acute lung injury (ALI), then routine mechanical ventilation (MV) for 8 h during which vaporized PFC inspiration was given for 2 h, and then killed to have the small intestine taken out, CMV group (n=8), undergoing routine MV for 8 h after the establishment of ALI model as MV control group, and sham operation group (n=2), undergoing only laparotomy to take out the small intestine. Apoptosis index of intestine mucosa was examined by TUNEL. Immunohistochemistry was used to detect the protein expression of BAX and bcl-2. RT-PCR was used to detect the mRNA expression of BAX and bcl-2.

RESULTS

The apoptotic cells were mainly distributed in the intestinal villi of the VMV and CMV groups, and only a few apoptotic cells could be seen in the sham operation group. The apoptosis rate of the VMV group was (22.9+/-2.3)%, significantly lower than that of the CMV group [(70.6+/-3.4)%, P<0.05]. The protein expression of BAX of the VMV group was significantly lower than that of the CMV group (P<0.05), and the protein expressing of bcl-2 of the VMV group was significantly higher than that of the CMV group (P<0.05). The mRNA expression of BAX of the VMV group was 7.10+/-0.32, significantly lower than that of the CMV group (9.29+/-1.06, P<0.05), and the MRNA expression of bcl-2 of the VMV group was 10.29+/-2.51, significantly higher than that of the CMV group (5.18+/-1.08, P<0.05). The mRNA expression and protein expression of BAX and bcl-2 were all at very low levels in the sham operating group.

CONCLUSION

PFC vaporized inspiration may reduce mucosa apoptosis of small intestine, with the possible mechanism of inhibiting the expression of the BAX gene and raising the expression of the bcl-2 gene.

摘要

目的

探讨汽化全氟碳（PFC）吸入对肠黏膜细胞凋亡的影响。

方法

将18只仔猪随机分为3组，VMV组（n = 8），经支气管内注入磺基琥珀酸二辛酯钠致急性肺损伤（ALI），然后进行常规机械通气（MV）8小时，其中给予汽化PFC吸入2小时，之后处死并取出小肠；CMV组（n = 8），在建立ALI模型后作为MV对照组进行常规MV 8小时；假手术组（n = 2），仅行剖腹术取出小肠。采用TUNEL法检测肠黏膜凋亡指数。免疫组织化学法检测BAX和bcl-2蛋白表达。RT-PCR法检测BAX和bcl-2 mRNA表达。

结果

凋亡细胞主要分布于VMV组和CMV组的肠绒毛，假手术组仅见少量凋亡细胞。VMV组凋亡率为（22.9±2.3）%，显著低于CMV组[（70.6±3.4）%，P<0.05]。VMV组BAX蛋白表达显著低于CMV组（P<0.05），VMV组bcl-2蛋白表达显著高于CMV组（P<0.05）。VMV组BAX mRNA表达为7.10±0.32，显著低于CMV组（9.29±1.06，P<0.05），VMV组bcl-2 mRNA表达为10.29±2.51，显著高于CMV组（5.18±1.08，P<0.05）。假手术组BAX和bcl-2的mRNA表达及蛋白表达均处于极低水平。