Xiao Wen-lin, Shi Bing, Huang Lei, Zheng Qian, Li Sheng, Lu Yong
Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Jan;37(1):137-40.
To modify the operation of dissecting embryonic palatal shelves and purify the mouse embryonic palatal mesenchymal (EPM) cells in primary culture.
The embryonic palatal shelves were dissected using a surgical microscope by modified operation. Then the embryonic palatal shelves were incubated with Dispase and the isolated EPM cells were cultured. Immunofluorescence technique was used to identify the characteristics of cells.
Embryonic palatal shelves could be dissected accurately and easily with a modified operation. The purified EPM cells contained scarcely epithelial cells. EPM cells were anti-HNK-1, S-100, vimentin positive and anti-CK negative.
A modified method for dissecting embryonic palatal shelves and purifying the EPM cells of primary culture was established.
改进胚胎腭突的解剖操作并纯化原代培养的小鼠胚胎腭间充质（EPM）细胞。
采用改良操作在手术显微镜下解剖胚胎腭突。然后用Dispase孵育胚胎腭突，分离得到的EPM细胞进行培养。采用免疫荧光技术鉴定细胞特性。
改良操作可准确、轻松地解剖胚胎腭突。纯化后的EPM细胞几乎不含上皮细胞。EPM细胞抗HNK-1、S-100、波形蛋白呈阳性，抗细胞角蛋白呈阴性。
建立了一种改良的胚胎腭突解剖方法及原代培养EPM细胞的纯化方法。
