Ohuchi N, Sato S, Akimoto M, Taira Y, Matoba N, Takahashi K, Mori S
Second Department of Surgery, Tohoku University School of Medicine, Sendai, Japan.
Jpn J Surg. 1991 Mar;21(2):129-37. doi: 10.1007/BF02470899.
The immunohistochemical expression of DF3 antigen and serum concentrations of CA15-3, a breast carcinoma-associated antigen recognized by the monoclonal antibodies (MAbs) 115D8 and DF3, was investigated in breast cancer patients. The levels of serum CA15-3 in 23 primary breast cancer patients did not correlate to the percentage cytoplasmic reactivity of MAb DF3 with the carcinoma cells in tissue specimens from each respective patient. In 17 patients who subsequently developed metastatic breast cancer, however, the serum CA15-3 concentrations generally correlated well to the cytoplasmic reactivity of MAb DF3 with the carcinoma cells in specimens obtained at initial biopsy or mastectomy. Elevated levels of serum CA15-3 were seen in metastatic breast cancer patients when the carcinoma cells in their primary specimens expressed enhanced levels of cytoplasmic DF3 antigen. The results of this study suggest that the immunohistochemical demonstration of DF3 antigen in tissue specimens, together with the periodical measurement of circulating CA15-3 antigen, may be important for monitoring the clinical course of breast cancer patients.
在乳腺癌患者中，研究了DF3抗原的免疫组化表达以及CA15-3的血清浓度，CA15-3是一种由单克隆抗体（MAb）115D8和DF3识别的乳腺癌相关抗原。23例原发性乳腺癌患者的血清CA15-3水平与各自患者组织标本中MAb DF3与癌细胞的细胞质反应百分比无关。然而，在随后发生转移性乳腺癌的17例患者中，血清CA15-3浓度通常与初次活检或乳房切除术时获得的标本中MAb DF3与癌细胞的细胞质反应性密切相关。当原发性标本中的癌细胞表达增强的细胞质DF3抗原水平时，转移性乳腺癌患者的血清CA15-3水平升高。本研究结果表明，组织标本中DF3抗原的免疫组化显示以及循环CA15-3抗原的定期测量可能对监测乳腺癌患者的临床病程很重要。
