De La Torre Anne, Debiton Eric, Juanéda Pierre, Durand Denys, Chardigny Jean-Michel, Barthomeuf Chantal, Bauchart Dominique, Gruffat Dominique
INRA, Unité de Recherche sur les Herbivores, Equipe Nutriments et Métabolismes, 63122 Saint Genès-Champanelle, France.
Br J Nutr. 2006 Feb;95(2):346-52. doi: 10.1079/bjn20051634.
Although many data are available concerning anticarcinogenic effects of industrial conjugated linoleic acid (CLA), few studies have reported the antitumour properties of CLA mixtures originating from ruminant products. The aim of the present study was to investigate the in vitro antiproliferative effects of beef CLA mixtures on breast, lung, colon, melanoma and ovarian human cancer cell lines. For this purpose, four fatty acid (FA) extracts prepared from beef lipid and varying in their CLA composition, their corresponding purified CLA-enriched fractions, and mixtures of pure synthetic CLA, the composition of which reproduced that of the four selected beef samples, were tested on cancer cell lines. Cancer cells were exposed for 48 h to medium containing 100 microm-FA and their proliferation was determined by quantifying cellular DNA content (Hoechst 33342 dye). Compared with cells incubated without FA, the number of cancer cells was reduced from 25 to 67 % (P<0.0001) following FA treatment. Antiproliferative effects of CLA mixtures varied in magnitude according to the source of FA, the CLA composition and the cell lines. CLA mixtures naturally present in beef inhibited the proliferation of human cancer cell lines, a high content in cis-trans isomers allowing the most important antiproliferative effect. Beef total FA exhibited a greater growth-inhibitory activity than their corresponding CLA-enriched fractions. These results suggested that either beef FA other than beef CLA could possess antiproliferative properties and/or the existence of complementary effects of non-conjugated FA and CLA, which could favour the antiproliferative properties of beef total FA.
尽管有许多关于工业共轭亚油酸（CLA）抗癌作用的数据，但很少有研究报道源自反刍动物产品的CLA混合物的抗肿瘤特性。本研究的目的是调查牛肉CLA混合物对人乳腺癌、肺癌、结肠癌、黑色素瘤和卵巢癌细胞系的体外抗增殖作用。为此，从牛肉脂质中制备了四种脂肪酸（FA）提取物，其CLA组成各不相同，以及相应的纯化CLA富集馏分，还有纯合成CLA的混合物（其组成再现了四个选定牛肉样品的组成），并在癌细胞系上进行了测试。将癌细胞暴露于含有100微摩尔FA的培养基中48小时，并通过定量细胞DNA含量（Hoechst 33342染料）来测定其增殖情况。与未用FA孵育的细胞相比，FA处理后癌细胞数量减少了25%至67%（P<0.0001）。CLA混合物的抗增殖作用根据FA来源、CLA组成和细胞系的不同而有所差异。牛肉中天然存在的CLA混合物抑制了人癌细胞系的增殖，顺反异构体的高含量产生了最重要的抗增殖作用。牛肉总FA表现出比其相应的CLA富集馏分更大的生长抑制活性。这些结果表明，牛肉CLA以外的牛肉FA可能具有抗增殖特性，和/或非共轭FA与CLA存在互补作用，这可能有利于牛肉总FA的抗增殖特性。
