Actis G C, Ponzetto A, D'Urso N, Saracco G, Crepaldi T, Bellone G, Catucci L M, Zarcone D, Verme G
Divisione di Gastroenterologia, Ospedale Molinette, Torino, Italy.
Liver. 1991 Apr;11(2):106-13. doi: 10.1111/j.1600-0676.1991.tb00500.x.
In a rapid 51Cr release assay, peripheral blood mononuclear cells from 12 healthy donors did not lyse the hepatitis B virus deoxyribonucleic-acid-transfected human hepatoma cell line 2.2.15, but under the same experimental conditions they did lyse K562 cells. Peripheral blood mononuclear cells from 10 out of 16 patients with chronic active hepatitis B exhibited cytotoxic activity against 2.2.15 cells in the presence of a relatively reduced natural killer cell activity to the K562 cell target. Enhancement of the cytotoxic activity to 2.2.15 cells was statistically significant in the group of patients being treated with leukocyte alpha-interferon. The activity was not influenced by the degree of human leukocyte antigen type matching between effector and target, and was enhanced by depletion of T-cells and by in vitro interferon treatment. These results therefore support the concept of a natural killer-like cell activated by clinical administration of interferon in chronic active hepatitis B patients. This cell effector was lytic for the virus B negative HEP-G2 cells also. However, T-cells purified from a few patients failed to lyse the HEP-G2 while lysing the 2.2.15 target, thus indicating that a preferential recognition of the virus-infected target may be exerted by certain T-lymphocyte subsets. The use of the human leukocyte antigen type defined, highly differentiated, hepatitis B virus releasing 2.2.15 cell line as target for fresh lymphocytes in this cytolytic assay did not disclose cytolytic T-cells in an obvious way. Further manipulation of this system perhaps using T-cell clones may be the next step to exploit the investigative possibilities offered by the availability of the 2.2.15 cell target.
在一项快速的51Cr释放试验中，来自12名健康供体的外周血单个核细胞不能裂解转染了乙型肝炎病毒脱氧核糖核酸的人肝癌细胞系2.2.15，但在相同实验条件下它们能裂解K562细胞。16例慢性活动性乙型肝炎患者中有10例的外周血单个核细胞在对K562细胞靶标的自然杀伤细胞活性相对降低的情况下，对2.2.15细胞表现出细胞毒活性。在接受白细胞α-干扰素治疗的患者组中，对2.2.15细胞的细胞毒活性增强具有统计学意义。该活性不受效应细胞与靶细胞之间人类白细胞抗原类型匹配程度的影响，并且通过T细胞耗竭和体外干扰素处理而增强。因此，这些结果支持在慢性活动性乙型肝炎患者中通过临床给予干扰素激活自然杀伤样细胞的概念。这种细胞效应物对乙型肝炎病毒阴性的HEP-G2细胞也具有裂解作用。然而，从少数患者中纯化的T细胞在裂解2.2.15靶标时未能裂解HEP-G2细胞，这表明某些T淋巴细胞亚群可能对病毒感染的靶标具有优先识别作用。在这种细胞溶解试验中，使用定义了人类白细胞抗原类型、高度分化、释放乙型肝炎病毒的2.2.15细胞系作为新鲜淋巴细胞的靶标，并未明显揭示细胞毒性T细胞。对该系统进行进一步操作，也许使用T细胞克隆，可能是利用2.2.15细胞靶标所提供的研究可能性的下一步。
