用多层磷酸钙-DNA纳米颗粒有效转染细胞。
Effective transfection of cells with multi-shell calcium phosphate-DNA nanoparticles.
作者信息
Sokolova Viktoriya V, Radtke Ina, Heumann Rolf, Epple Matthias
机构信息
Institute of Inorganic Chemistry, University of Duisburg-Essen, Universitaetsstrasse 5-7, D-45117 Essen, Germany.
出版信息
Biomaterials. 2006 Jun;27(16):3147-53. doi: 10.1016/j.biomaterials.2005.12.030.
Coated calcium phosphate nanoparticles were prepared for cell transfection. A calcium phosphate nanoparticle served as core which was then coated with DNA for colloidal stabilisation. The efficiency of transfection could be considerably increased by adding another layer of calcium phosphate on the surface, thereby incorporating DNA into the particle and preventing its degradation within the cell by lysosomes. A subsequent outermost layer of DNA on the calcium phosphate gave a colloidal stabilisation. The efficiency of such multi-shell particles was significantly higher than that of simple DNA-coated calcium phosphate nanoparticles. The transfection efficiency of EGFP-encoding DNA was tested with different cell lines (T-HUVEC, HeLa, and LTK). The dispersions were stable and could be used for transfection after 2 weeks of storage at 4 degrees C without loss of efficiency.
制备了用于细胞转染的磷酸钙包被纳米颗粒。以磷酸钙纳米颗粒为核心，然后用DNA进行包被以实现胶体稳定。通过在表面添加另一层磷酸钙，可以显著提高转染效率，从而将DNA整合到颗粒中，并防止其在细胞内被溶酶体降解。在磷酸钙上随后形成的最外层DNA提供了胶体稳定性。这种多壳颗粒的效率明显高于简单的DNA包被磷酸钙纳米颗粒。用不同的细胞系（T-HUVEC、HeLa和LTK）测试了编码EGFP的DNA的转染效率。分散体是稳定的，在4℃储存2周后可用于转染，且效率不会损失。
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