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[Molecular cloning of a novel low-molecular-weight glutenin subunit gene from wheat variety "Chuanmai 42"].

作者信息

Shen Lei, Long Hai, Yan Ze-Hong, Wei Yu-Ming, Zheng You-Liang

机构信息

Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, China.

出版信息

Yi Chuan. 2006 Jan;28(1):57-64.

PMID:16469718
Abstract

Using PCR-amplification method, the coding sequences of a novel low-molecular-weight glutenin (LMW-GS) gene, LMWCM42-1, was isolated from the genomic DNA of wheat (Triticum aestivum L.) variety 'Chuanmai 42'. The full coding region of this gene consisted of 846 nucleotides, and encoded a protein with 281 amino acids. LMWCM42-1 is a typical low-molecular-weight glutenin subunit (LMW-GS) gene. In spite of high similarity with the known LMW-GSs, LMWCM42-1 was quite different from the known LMW-GS in the N-terminal, central repetitive domain and C-terminal domain. It was found that LMWCM42-1 was located within the Glu-D3 locus by phylogenetic analysis.

摘要

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