Zeng Jie, Dai Shou-Fen, Zheng You-Liang, Liu Deng-Cai, Wei Yu-Ming, Yan Ze-Hong
Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, China.
Yi Chuan. 2008 May;30(5):633-41. doi: 10.3724/sp.j.1005.2008.00633.
More and more low-molecular-weight glutenin(LMW glutenin) genes were isolated and characterized from hexaploid wheat (Triticum aestivum L.). However, few homologous genes were obtained from its relative species, which limited our understanding of the relationships among them. Therefore, it is necessary to isolate LMW glutenin homologous genes from wheat wild relative species. Using a pair of specific oligonucleotide PCR primers for Taenitherum genomic DNA, a LMW glutenin gene sequence, with nucleotide sequence in 1 035 bp and deduced amino acid sequence with 343 amino acid residues, was obtained. This sequence was a typical LMW glutenin sequence and characterized by a signal peptide of 21 amino acid residues, a N-terminal conservative domain of 13 amino acid residues, a repetitive domain of short peptide, and a C-terminal conservative domain. Sequence alignment showed the main differences and the relationships between LMW glutenin genes from wheat and Taenitherum. The results presented here give a reference to isolate LMW glutenin gene from Taenitherum, as well as other wheat wild relatives.
越来越多的低分子量麦谷蛋白（LMW麦谷蛋白）基因从小麦（Triticum aestivum L.）中被分离和鉴定。然而，从其近缘物种中获得的同源基因很少，这限制了我们对它们之间关系的理解。因此，有必要从小麦野生近缘物种中分离LMW麦谷蛋白同源基因。使用一对针对Taenitherum基因组DNA的特异性寡核苷酸PCR引物，获得了一个LMW麦谷蛋白基因序列，其核苷酸序列为1035bp，推导的氨基酸序列有343个氨基酸残基。该序列是典型的LMW麦谷蛋白序列，其特征在于有一个21个氨基酸残基的信号肽、一个13个氨基酸残基的N端保守结构域、一个短肽重复结构域和一个C端保守结构域。序列比对显示了小麦和Taenitherum的LMW麦谷蛋白基因之间的主要差异和关系。本文的结果为从Taenitherum以及其他小麦野生近缘物种中分离LMW麦谷蛋白基因提供了参考。
