Lin Xiuli, Zhao Minggang, Qi Xinyu, Zhu Chenfu, Hao Aiyou
School of Pharmacy, Shandong University, Jinan, PR China.
Electrophoresis. 2006 Feb;27(4):872-9. doi: 10.1002/elps.200500657.
A charged highly water-soluble CD derivative, 6-O-(2-hydroxy-3-trimethylammoniopropyl)-beta-CD (herein noted as 6-HPTMA-beta-CD) was synthesized and successfully used as a chiral selector for enantiomeric separation of some acidic compounds by CZE in an uncoated capillary. Substitution with 2-hydroxy-3-trimethylammoniopropyl groups at the primary hydroxyl group of the CD was aimed at influencing the magnitude and selectivity of analyte-CD interactions. The behavior of 6-HPTMA-beta-CD was compared with that of the commercially available quaternary ammonium-beta-CD (QA-beta-CD) under the same separating conditions. The experiments were carried out using a BGE consisting of 50 mM phosphate in the pH range of 4-6 by adding a relatively low concentration of chiral selector (less than 10 mM). The effects of the concentration of CD and the pH of the electrolyte on the resolution of these compounds were studied.
合成了一种带电荷的高水溶性环糊精衍生物，6-O-(2-羟基-3-三甲基铵丙基)-β-环糊精（在此记为6-HPTMA-β-CD），并成功用作手性选择剂，通过未涂层毛细管中的毛细管区带电泳法对一些酸性化合物进行对映体分离。在环糊精的伯羟基上用2-羟基-3-三甲基铵丙基进行取代，目的是影响分析物与环糊精相互作用的强度和选择性。在相同的分离条件下，将6-HPTMA-β-CD的行为与市售的季铵化-β-环糊精（QA-β-CD）进行了比较。通过添加相对低浓度的手性选择剂（小于10 mM），使用pH范围为4至6的50 mM磷酸盐组成的背景电解质进行实验。研究了环糊精浓度和电解质pH对这些化合物分离度的影响。
