Zhong W, Wang Q T, Sun T, Wang F, Liu J, Leach R, Johnson A, Puscheck E E, Rappolee D A
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
Mol Reprod Dev. 2006 May;73(5):540-50. doi: 10.1002/mrd.20417.
Signaling by fibroblast growth factor (FGF) is essential is for trophoblast stem (TS) cells and preimplantation embryos. FGF4 provides essential signaling, but the expression of the complete set of 23 FGF family members has not been analyzed. Here, semi-quantitative RT-PCR and microarray analyses were used to define expression of all FGF ligand mRNA. RT-PCR was done for developmentally important FGF subfamilies, FGF10/FGF22 and FGF8/FGF17/FGF18 as well as FGF11. FGF4 and FGF18 are detected at highest levels by RT-PCR and microarrays. FGF10 was detected at low levels in both assays. FGF11 was detected at moderate levels by microarray, but not by RT-PCR. FGF17 was detected at low levels by array and moderate levels by RT-PCR. FGF8 and FGF22 were detected by RT-PCR, but not by microarrays during late cleavage divisions. FGF8, FGF5, and FGF9 were detected in the oocyte by microarray. FGF2, FGF3, and FGF7 were not detected by RT-PCR or microarrays and FGF13, FGF14, and FGF23 were not detected by microarray. Since a major role of FGF is to maintain TS cells, we tested human and mouse placental cell lines and early gestation human placenta for expression of FGF ligands. Expression in mouse TS cells was compared with preimplantation embryos, and human placental cell line expression was compared with human placenta, to infer which ligands are expressed in placental lineage vs. other cell lineages. The data suggest that human and mouse placenta share FGF18 and its high expression suggests preimplantation and early placental function.
成纤维细胞生长因子（FGF）信号传导对于滋养层干细胞（TS）和植入前胚胎至关重要。FGF4提供关键信号，但尚未对23个FGF家族成员的完整表达情况进行分析。在此，运用半定量逆转录聚合酶链反应（RT-PCR）和微阵列分析来确定所有FGF配体mRNA的表达。针对发育中重要的FGF亚家族、FGF10/FGF22和FGF8/FGF17/FGF18以及FGF11进行了RT-PCR。通过RT-PCR和微阵列检测发现FGF4和FGF18的表达水平最高。在两种检测方法中，FGF10的检测水平都很低。微阵列检测到FGF11的表达水平中等，但RT-PCR未检测到。微阵列检测到FGF1十七的表达水平很低，而RT-PCR检测到其表达水平中等。RT-PCR检测到了FGF8和FGF22，但在晚期卵裂期微阵列未检测到。微阵列在卵母细胞中检测到了FGF8、FGF5和FGF9。RT-PCR和微阵列均未检测到FGF2、FGF3和FGF7，微阵列未检测到FGF13、FGF14和FGF23。由于FGF的主要作用是维持TS细胞，我们检测了人和小鼠胎盘细胞系以及妊娠早期人胎盘的FGF配体表达。将小鼠TS细胞中的表达与植入前胚胎进行比较，将人胎盘细胞系的表达与人胎盘进行比较，以推断哪些配体在胎盘谱系与其他细胞谱系中表达。数据表明，人和小鼠胎盘都有FGF18表达，其高表达提示植入前和早期胎盘功能。
