Department of Physiology, Wayne State University, Detroit, Michigan, United States of America.
PLoS One. 2012;7(9):e43239. doi: 10.1371/journal.pone.0043239. Epub 2012 Sep 18.
Numerous studies have focused on the transcriptional signatures that underlie the maintenance of embryonic stem cell (ESC) pluripotency. However, it remains unclear whether ESC retain transcriptional aberrations seen in in vitro cultured embryos. Here we report the first global transcriptional profile comparison between ESC generated from either in vitro cultured or in vivo derived primate embryos by microarray analysis. Genes involved in pluripotency, oxygen regulation and the cell cycle were downregulated in rhesus ESC generated from in vitro cultured embryos (in vitro ESC). Significantly, several gene differences are similarly downregulated in preimplantation embryos cultured in vitro, which have been associated with long term developmental consequences and disease predisposition. This data indicates that prior to derivation, embryo quality may influence the molecular signature of ESC lines, and may differentially impact the physiology of cells prior to or following differentiation.
许多研究都集中在胚胎干细胞（ESC）多能性维持的转录特征上。然而，目前尚不清楚 ESC 是否保留了体外培养胚胎中出现的转录异常。在这里，我们通过微阵列分析报告了首次在体外培养或体内衍生的灵长类胚胎来源的 ESC 之间的全基因组转录谱比较。参与多能性、氧调节和细胞周期的基因在体外培养胚胎来源的恒河猴 ESC（in vitro ESC）中下调。值得注意的是，体外培养的胚胎中也有几个基因差异下调，这些差异与长期发育后果和疾病易感性有关。这些数据表明，在衍生之前，胚胎质量可能会影响 ESC 系的分子特征，并可能在分化之前或之后对细胞的生理学产生不同的影响。
