Basaraba Randall J, Dailey Deanna D, McFarland Christine T, Shanley Crystal A, Smith Erin E, McMurray David N, Orme Ian M
Mycobacteria Research Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Tuberculosis (Edinb). 2006 Sep;86(5):386-94. doi: 10.1016/j.tube.2005.11.003. Epub 2006 Feb 10.
Guinea pigs infected by low dose aerosol with the H37Rv strain of Mycobacterium tuberculosis rapidly developed granulomatous lesions in the pulmonary parenchyma and within the intra-thoracic hilar lymph node cluster. Lung lesions showed no predilection for specific lobes and were perivascular, peribronchial and peribronchiolar throughout the subpleural, hilar and pulmonary parenchyma. Marked hilar lymph node enlargement was due to coalescing foci of subcapsular, paracortical and medullary granulomatous inflammation that progressed to necrosis that effaced normal lymph node architecture. Lymph node lesions became severe and progressed more rapidly than pulmonary lesions. Immunization with BCG 6 weeks prior to infection significantly reduced the lung and lymph node lesion burden as well as the progression to necrosis in both tissues. Lymph node inflammation in BCG immunized animals partially resolved and was replaced by fibroblasts and fibrous connective tissue while lesions from non-immunized animals continued to progress to necrosis. We discuss here the observation that the distribution and progression of lung and lymph node lesions in the guinea pig aerosol model of tuberculosis have considerable similarity to the naturally occurring disease in children.
用低剂量气溶胶感染结核分枝杆菌H37Rv菌株的豚鼠，肺部实质和胸内肺门淋巴结簇迅速出现肉芽肿性病变。肺部病变无特定肺叶偏好，在胸膜下、肺门和肺实质内均为血管周围、支气管周围和细支气管周围病变。明显的肺门淋巴结肿大是由于包膜下、皮质旁和髓质肉芽肿性炎症融合病灶进展为坏死，破坏了正常淋巴结结构。淋巴结病变比肺部病变更严重且进展更快。感染前6周用卡介苗免疫可显著减轻肺和淋巴结病变负担以及两个组织中坏死的进展。卡介苗免疫动物的淋巴结炎症部分消退，被成纤维细胞和纤维结缔组织取代，而未免疫动物的病变继续进展为坏死。我们在此讨论豚鼠气溶胶结核模型中肺和淋巴结病变的分布及进展与儿童自然发生疾病有相当大相似性的观察结果。
