Bilang R, Iida S, Peterhans A, Potrykus I, Paszkowski J
Institute of Plant Sciences, ETH Zentrum, Zürich, Switzerland.
Gene. 1991 Apr;100:247-50. doi: 10.1016/0378-1119(91)90375-l.
We have modified the 3'-coding region of the hygromycin B (Hy) phosphotransferase-encoding gene (hph). The level of Hy resistance conferred by the modified hph genes was determined in Escherichia coli and Nicotiana tabacum. Polypeptides up to 12 amino acids shorter or longer than the wild-type (wt) protein remained active in both pro- and eukaryotic cells. A gene construct of wt length, but with a modified 3' sequence, was less active as measured by the level of Hy resistance. Therefore, the nature of the C terminus of the hph gene product, rather than the length of the polypeptide, influences the ability to confer resistance to Hy.
我们对潮霉素B（Hy）磷酸转移酶编码基因（hph）的3'编码区进行了修饰。在大肠杆菌和烟草中测定了修饰后的hph基因赋予的Hy抗性水平。比野生型（wt）蛋白短或长多达12个氨基酸的多肽在原核细胞和真核细胞中均保持活性。通过Hy抗性水平测定，野生型长度但3'序列修饰的基因构建体活性较低。因此，hph基因产物C末端的性质而非多肽长度影响赋予Hy抗性的能力。
