基于潮霉素抗性的裂殖酵母启动子探针载体。

Fission yeast promoter-probe vectors based on hygromycin resistance.

作者信息

Burland T G, Pallotta D, Tardif M C, Lemieux G, Dove W F

机构信息

McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.

出版信息

Gene. 1991 Apr;100:241-5. doi: 10.1016/0378-1119(91)90374-k.

DOI:10.1016/0378-1119(91)90374-k

PMID:1647355

Abstract

We have constructed fission yeast vectors that carry either complete or 5'-truncated alleles of the hph gene, encoding hygromycin B phosphotransferase. We show that plasmid-borne hph can be expressed in fission yeast to confer hygromycin resistance. The vectors permit selection or screening in fission yeast for promoter activity of DNA fragments from other species. We used the vectors to identify several genomic sequences from Physarum that provide promoter function in fission yeast.

摘要

我们构建了携带hph基因完整或5'端截短等位基因的裂殖酵母载体，该基因编码潮霉素B磷酸转移酶。我们发现质粒携带的hph基因能够在裂殖酵母中表达，赋予其潮霉素抗性。这些载体可用于在裂殖酵母中选择或筛选来自其他物种的DNA片段的启动子活性。我们利用这些载体鉴定出了来自黏菌的几个基因组序列，这些序列在裂殖酵母中具有启动子功能。