Elton D M, Halliburton I W, Killington R A, Meredith D M, Bonass W A
Department of Microbiology, University of Leeds, U.K.
Gene. 1991 May 30;101(2):203-8. doi: 10.1016/0378-1119(91)90412-5.
To localize gene that may encode immunogens potentially important for recombinant vaccine design, we have analysed a region of the equine herpesvirus type-1 (EHV-1) genome where a glycoprotein-encoding gene had previously been mapped. The 4707-bp BamHI-EcoRI fragment from the short unique region of the EHV-1 genome was sequenced. This sequence contains three entire open reading frames (ORFs), and portions of two more. ORF1 codes for 161 amino acids (aa), and represents the C terminus of a possible membrane-bound protein. ORF2 (424 aa) and ORF3 (550 aa) are potential glycoprotein-encoding genes; the predicted aa sequences contain possible signal sequences, N-linked glycosylation sites and transmembrane domains; they also show homology to the glycoproteins gI and gE of herpes simplex virus type-1 (HSV-1), and the related proteins of pseudorabies virus and varicella-zoster virus. The predicted aa sequence of ORF4 shares no homology with other known herpesvirus proteins, but the nucleotide sequence shows a high level of homology with the corresponding region of the EHV-4 genome. ORF5 may be related to US9 of HSV-1.
为了定位可能编码对重组疫苗设计具有潜在重要性的免疫原的基因，我们分析了1型马疱疹病毒（EHV-1）基因组的一个区域，之前已在该区域定位了一个糖蛋白编码基因。对来自EHV-1基因组短独特区域的4707 bp BamHI-EcoRI片段进行了测序。该序列包含三个完整的开放阅读框（ORF），以及另外两个的部分。ORF1编码161个氨基酸（aa），代表一种可能的膜结合蛋白的C末端。ORF2（424 aa）和ORF3（550 aa）是潜在的糖蛋白编码基因；预测的氨基酸序列包含可能的信号序列、N-连接糖基化位点和跨膜结构域；它们还与1型单纯疱疹病毒（HSV-1）的糖蛋白gI和gE以及伪狂犬病病毒和水痘-带状疱疹病毒的相关蛋白具有同源性。ORF4的预测氨基酸序列与其他已知的疱疹病毒蛋白没有同源性，但核苷酸序列与EHV-4基因组的相应区域具有高度同源性。ORF5可能与HSV-1的US9相关。
