Audonnet J C, Winslow J, Allen G, Paoletti E
Virogenetics Corporation, Rensselaer Technology Park, Troy, New York 12180-8349.
J Gen Virol. 1990 Dec;71 ( Pt 12):2969-78. doi: 10.1099/0022-1317-71-12-2969.
The nucleotide sequence of a 6.4 kbp portion of the 10.6 kbp BamHI fragment D contained in the unique short region of the equine herpesvirus type 1 (EHV-1) genome has been determined. Analysis of this sequence revealed five open reading frames (ORFs), four complete and one incomplete, which were encoded by the same sense strand. Comparison of the EHV-1 DNA sequence with that encoding glycoproteins of other alphaherpesviruses has revealed no significant homologies. Comparison at the amino acid level, however, has demonstrated regions of significant sequence similarity between the three complete EHV-1 ORFs 2, 3 and 4, and the herpes simplex virus type 1 (HSV-1) glycoprotein gD encoded by the US6 gene, the HSV-1 glycoprotein gI encoded by the US7 gene and the HSV-1 glycoprotein gE encoded by the US8 gene, respectively. The interrupted ORF 5 was found to display partial homology with the HSV-1 US9-encoded protein, but no homology was found between the protein encoded by ORF 1 and other proteins. The three collinear EHV-1 ORFs encoding putative glycoproteins with homology to the HSV-1 glycoproteins were therefore designated EHV-1 gD, gI and gE, respectively. Moreover, further similarities were found between EHV-1 gD and pseudorabies virus (PRV) gp50, between EHV-1 gI and PRV gp63 and varicellazoster virus (VZV) gpIV, and between EHV-1 gE and PRV gI and VZV gpI. It is concluded that EHV-1, PRV, HSV-1 and VZV encode homologous glycoprotein genes in the small unique components of their genomes and that the genetic organization of these regions is conserved.
已确定马疱疹病毒1型（EHV-1）基因组独特短区域中包含的10.6 kbp BamHI片段D的6.4 kbp部分的核苷酸序列。对该序列的分析揭示了五个开放阅读框（ORF），其中四个完整，一个不完整，它们由同一条有义链编码。将EHV-1 DNA序列与编码其他α疱疹病毒糖蛋白的序列进行比较，未发现明显的同源性。然而，在氨基酸水平上的比较表明，EHV-1的三个完整ORF 2、3和4与单纯疱疹病毒1型（HSV-1）由US6基因编码的糖蛋白gD、由US7基因编码的糖蛋白gI和由US8基因编码的糖蛋白gE之间存在显著的序列相似区域。发现中断的ORF 5与HSV-1 US9编码的蛋白显示部分同源性，但在ORF 1编码的蛋白与其他蛋白之间未发现同源性。因此，将与HSV-1糖蛋白具有同源性的三个共线EHV-1 ORF分别命名为EHV-1 gD、gI和gE。此外，还发现EHV-1 gD与伪狂犬病病毒（PRV）gp50之间、EHV-1 gI与PRV gp63和水痘带状疱疹病毒（VZV）gpIV之间以及EHV-1 gE与PRV gI和VZV gpI之间存在进一步的相似性。得出的结论是，EHV-1、PRV、HSV-1和VZV在其基因组的小独特组分中编码同源糖蛋白基因，并且这些区域的遗传组织是保守的。
