Kohno-Murase Junko, Iwabuchi Mari, Endo-Kasahara Saori, Sugita Koichi, Ebinuma Hiroyasu, Imamura Jun
Plantech Research Institute, 227-0033, Yokohama, Japan.
Transgenic Res. 2006 Feb;15(1):95-100. doi: 10.1007/s11248-005-3736-8.
Conjugated linoleic acid (CLA) has anti-carcinogenic and anti-atherosclerosis activity, and modulatory effects on the immune system and lipid metabolism. To produce a transgenic rice plant that can accumulate CLA, a linoleate isomerase gene that can convert linoleic acid to trans-10, cis-12 CLA was introduced and expressed under the control of seed-specific promoters from the oleosin and globulin genes. The fatty acid composition of the transgenic rice grain was analyzed by gas chromatography. Although there was no clear difference in the fatty acid composition between seeds from transformed versus untransformed plants, a peak of trans-10, cis-12 CLA methyl ester, which was not present in seeds from untransformed plants, was found in transformed plants. The trans-10, cis-12 CLA comprised an average of 1.3% (w/w) of the total fatty acids in seeds carrying the oleosin promoter in comparison to 0.01% (w/w) in seeds carrying the globulin promoter. In addition, approximately 70 and 28% of the total amount of the CLA isomer were present in the triacylglycerol and free fatty acid fractions, respectively. These results demonstrate the ability to produce fatty acid components of vegetable oils with novel physiological activities in crops.
共轭亚油酸（CLA）具有抗癌和抗动脉粥样硬化活性，对免疫系统和脂质代谢具有调节作用。为了培育出能够积累CLA的转基因水稻植株，导入了一种可将亚油酸转化为反式-10，顺式-12 CLA的亚油酸异构酶基因，并在油质蛋白和球蛋白基因的种子特异性启动子控制下进行表达。通过气相色谱法分析转基因水稻籽粒的脂肪酸组成。虽然转化植株与未转化植株种子的脂肪酸组成没有明显差异，但在转化植株中发现了反式-10，顺式-12 CLA甲酯的峰，而未转化植株种子中不存在该峰。与携带球蛋白启动子的种子中0.01%（w/w）相比，携带油质蛋白启动子的种子中反式-10，顺式-12 CLA平均占总脂肪酸的1.3%（w/w）。此外，CLA异构体总量的约70%和28%分别存在于三酰甘油和游离脂肪酸组分中。这些结果证明了在作物中生产具有新生理活性的植物油脂肪酸成分的能力。
