Lin Lu, Sun Mei-Li, Wang Kaifeng, Gao Jian, Ji Xiao-Jun, Zhao Quanyu
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, People's Republic of China.
School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, 224051, People's Republic of China.
Bioresour Bioprocess. 2025 Jul 11;12(1):72. doi: 10.1186/s40643-025-00911-7.
Conjugated linoleic acid (CLA) has received much attention for its beneficial physiological effects, particularly anticancer and metabolic control activity. However, traditional sources rely on extraction and chemical synthesis, both of which are not commercially viable. This article describes the research progress on sustainable alternative biosynthesis methods for the production of CLA, which is an attractive approach for commercial production because of its high stereoselectivity and convenient purification process. Here, we offer a comprehensive review of the current state and research progress in the biosynthesis of CLA, including production strains, biosynthesis mechanisms and improvements in the biosynthesis of CLA by genetic engineering. Finally, future research directions for improving the biosynthesis of CLA are defined in the light of recent advances, barriers and trends in this field.
共轭亚油酸（CLA）因其有益的生理效应，特别是抗癌和代谢控制活性而备受关注。然而，传统来源依赖于提取和化学合成，这两种方法在商业上都不可行。本文描述了用于生产CLA的可持续替代生物合成方法的研究进展，由于其高立体选择性和便捷的纯化过程，这是一种具有吸引力的商业生产方法。在此，我们全面综述了CLA生物合成的现状和研究进展，包括生产菌株、生物合成机制以及通过基因工程改进CLA的生物合成。最后，根据该领域的最新进展、障碍和趋势，确定了改善CLA生物合成的未来研究方向。
