Yildirim S, Yapar M, Kubar A
Selçuk University, Faculty of Dentistry, Department of Pediatric Dentistry, Konya, Turkey.
Oral Microbiol Immunol. 2006 Apr;21(2):73-8. doi: 10.1111/j.1399-302X.2006.00250.x.
BACKGROUND/AIMS: Kostmann syndrome, or severe congenital neutropenia, is an autosomal recessive disease of neutrophil production and is associated with severe periodontal pathology. The aim of this study was to determine whether human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) contribute to the pathogenesis of Kostmann syndrome periodontitis.
Supragingival plaque and saliva samples were taken from a 6-year-old boy and his 3-year-old sister suffering from Kostmann syndrome, and from two age- and gender-matched healthy children serving as controls. The samples were taken before and 24 months after periodontal treatment. Real-time polymerase chain reaction (TaqMan Real-Time PCR) assay was used to quantify HCMV and EBV DNA.
EBV was detected in baseline samples from the Kostmann syndrome patients but not in samples from the healthy control subjects. HCMV was only detected in the saliva of the boy with Kostman syndrome at baseline. Herpesviruses numbers decreased dramatically in the post-treatment samples.
EBV and HCMV were detected in the two subjects with Kostmann syndrome periodontitis. The results of the study indicate that nonsurgical treatment of Kostmann syndrome periodontitis can reduce supragingival and salivary herpes viral loads.
背景/目的：科斯特曼综合征，即严重先天性中性粒细胞减少症，是一种中性粒细胞生成的常染色体隐性疾病，与严重的牙周病变相关。本研究的目的是确定人巨细胞病毒（HCMV）和爱泼斯坦-巴尔病毒（EBV）是否在科斯特曼综合征牙周炎的发病机制中起作用。
从一名患有科斯特曼综合征的6岁男孩及其3岁妹妹以及两名年龄和性别匹配的健康儿童（作为对照）采集龈上菌斑和唾液样本。样本在牙周治疗前和治疗后24个月采集。采用实时聚合酶链反应（TaqMan实时PCR）检测法对HCMV和EBV DNA进行定量。
在科斯特曼综合征患者的基线样本中检测到EBV，但在健康对照受试者的样本中未检测到。仅在患有科斯特曼综合征的男孩基线唾液中检测到HCMV。治疗后样本中的疱疹病毒数量显著下降。
在两名患有科斯特曼综合征牙周炎的受试者中检测到EBV和HCMV。研究结果表明，科斯特曼综合征牙周炎的非手术治疗可降低龈上和唾液疱疹病毒载量。
