Rosen Noah A, Hopf Harriet W, Hunt Thomas K
Department of Surgery, Boston University Medical Center, Boston, Massachusetts, USA.
Wound Repair Regen. 2006 Jan-Feb;14(1):55-60. doi: 10.1111/j.1743-6109.2005.00088.x.
Oxygen plays a central role in wound healing. The hypothesis of this study was that the administration of a perfluorocarbon emulsion, which dissolves oxygen will increase subcutaneous tissue oxygen tension (PsqO2) in normovolemic and hemorrhaged animals. In the first set of experiments, PsqO2 was measured with a polarographic oxygen electrode along the dorsum of normovolemic-anesthetized rats (n=20) breathing supplemental oxygen. After baseline equilibration of tissue oxygen tension, perflubron emulsion (n=12) or saline control (n=8) was administered. Perflubron administration increased PsqO2 by 32.0 +/- 7.2 mmHg, whereas saline administration had no effect (-1.0 +/- 6.6 mmHg). In a second set of experiments, PsqO2 (n=12) was measured in rats breathing 100 percent oxygen after being bled 20 percent of their blood volume. Creating hypovolemic rats allowed for the study of the effect of perflubron emulsion administration on subcutaneous oxygen tension in tissues with compromised blood flow. Perflubron emulsion was only effective at increasing oxygen tension in tissues with oxygen extractions less then 2.0 vol%. These findings agree with those results analytically predicted based on the oxygen solubility of the perflubron emulsion.
氧气在伤口愈合中起着核心作用。本研究的假设是，给予能溶解氧气的全氟碳乳剂，将增加血容量正常和出血动物的皮下组织氧张力（PsqO2）。在第一组实验中，使用极谱氧电极沿着呼吸补充氧气的血容量正常且麻醉的大鼠（n = 20）的背部测量PsqO2。在组织氧张力达到基线平衡后，给予全氟溴辛烷乳剂（n = 12）或生理盐水对照（n = 8）。给予全氟溴辛烷使PsqO2增加了32.0±7.2 mmHg，而给予生理盐水则无影响（-1.0±6.6 mmHg）。在第二组实验中，对失血20%血容量后呼吸100%氧气的大鼠（n = 12）测量PsqO2。制造低血容量大鼠有助于研究给予全氟碳乳剂对血流受损组织中皮下氧张力的影响。全氟碳乳剂仅在氧摄取量低于2.0 vol%的组织中能有效增加氧张力。这些发现与基于全氟碳乳剂氧溶解度通过分析预测的结果一致。
