Colwell Amy S, Krummel Thomas M, Kong Wuyi, Longaker Michael T, Lorenz H Peter
Department of Surgery, Division of Plastic Surgery, Pediatric Surgery Research Laboratory, Stanford University Medical School, Stanford, California 94305-5148, USA.
Wound Repair Regen. 2006 Jan-Feb;14(1):81-90. doi: 10.1111/j.1524-475X.2005.00092.x.
Adult MRL/MpJ mice regenerate cartilage during repair of through-and-through ear punch wounds. However, the ability of this mouse strain to heal isolated cutaneous wounds by regeneration or with scar is unknown. The purpose of this study was to characterize the rate of reepithelialization and collagen architecture in dermal wounds from MRL/MpJ mice compared with C57bl/6 and Balb/c strains. Full-thickness incisional (5 mm) and excisional (2 mm diameter) skin wounds were made on the dorsum of 7-week-old MRL/MpJ, C57bl/6, and Balb/c mice. Ear punch wounds were made simultaneously on each animal. Reepithelialization was complete by 48 hours for incisional skin wounds in each strain. All excisional wounds showed incomplete reepithelialization at 24, 48, and 72 hours. At 14 days, all skin wounds had grossly healed. In contrast to the ear wounds made in C57bl/6 and Balb/c mice, MRL/MpJ ear wounds were completely healed by day 28. Dorsal skin wound sections at 14 and 28 days revealed dense collagen deposition and similar degrees of fibrosis between the three strains of mice. In conclusion, in contrast to wound healing in the ear, MRL/MpJ mouse dorsal cutaneous wounds heal similarly to C57bl/6 and Balb/c mice with dermal collagen deposition and scar formation.
成年MRL/MpJ小鼠在贯通性耳打孔伤口修复过程中可实现软骨再生。然而，该小鼠品系通过再生或形成瘢痕来愈合孤立性皮肤伤口的能力尚不清楚。本研究的目的是比较MRL/MpJ小鼠与C57bl/6和Balb/c品系小鼠真皮伤口的上皮再形成速率和胶原结构。在7周龄的MRL/MpJ、C57bl/6和Balb/c小鼠背部制作全层切开（5mm）和切除（直径2mm）皮肤伤口。同时在每只动物身上制作耳打孔伤口。各品系切开皮肤伤口在48小时时上皮再形成完成。所有切除伤口在24、48和72小时时均显示上皮再形成不完全。在14天时，所有皮肤伤口均已大体愈合。与C57bl/6和Balb/c小鼠的耳部伤口不同，MRL/MpJ耳部伤口在28天时完全愈合。14天和28天时的背部皮肤伤口切片显示，三种品系小鼠之间均有致密的胶原沉积和相似程度的纤维化。总之，与耳部伤口愈合不同，MRL/MpJ小鼠背部皮肤伤口的愈合方式与C57bl/6和Balb/c小鼠相似，会形成真皮胶原沉积和瘢痕。
