Sadaoka Tomohiko, Yamanishi Koichi, Mori Yasuko
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
J Gen Virol. 2006 Mar;87(Pt 3):501-508. doi: 10.1099/vir.0.81374-0.
The function of the human herpesvirus 7 (HHV-7) U47 gene, which is a positional homologue of the genes encoding glycoprotein O (gO) in human cytomegalovirus (HCMV) and human herpesvirus 6 (HHV-6), was analysed. A monoclonal antibody (mAb) against the U47 gene product reacted in immunoblots with proteins migrating at 49 and 51 kDa in lysates of HHV-7-infected cells and with 49 and 51 kDa proteins in partially purified virions. Digestion of the 49 and 51 kDa proteins with endoglycosidase H and peptide N-glycosidase F indicated that the U47-encoded proteins were modified with N-linked oligosaccharides. Therefore, the U47 gene and its product were named gO, as in HCMV and HHV-6. In addition, the anti-gO mAb co-immunoprecipitated glycoprotein H (gH) in HHV-7-infected cells, indicating an association between HHV-7 gO and gH. The results suggest that the HHV-7 gO-gH complex might have a similar function to that in HCMV or HHV-6, such as cell-cell fusion in virus infection.
对人类疱疹病毒7型（HHV-7）U47基因的功能进行了分析，该基因是人类巨细胞病毒（HCMV）和人类疱疹病毒6型（HHV-6）中编码糖蛋白O（gO）的基因的位置同源物。一种针对U47基因产物的单克隆抗体（mAb）在免疫印迹中与HHV-7感染细胞裂解物中迁移率为49 kDa和51 kDa的蛋白质以及部分纯化病毒粒子中的49 kDa和51 kDa蛋白质发生反应。用内切糖苷酶H和肽N-糖苷酶F对49 kDa和51 kDa蛋白质进行消化表明，U47编码的蛋白质被N-连接寡糖修饰。因此，如同在HCMV和HHV-6中一样，U47基因及其产物被命名为gO。此外，抗gO mAb在HHV-7感染细胞中共免疫沉淀了糖蛋白H（gH），表明HHV-7 gO与gH之间存在关联。结果表明，HHV-7 gO-gH复合物可能具有与HCMV或HHV-6中类似的功能，如病毒感染中的细胞间融合。
