Peng Shi-Qing, Huang Dong-Fen
State Key Laboratory of Tropical Crop Biotechnology, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Feb;32(1):52-6.
The actin-depolymerizing factor 4 gene (ADF4) of Arabidopsis thaliana was cloned and sequenced (Figs.1, 2). The plant expression vector with ADF4 was constructed and transformed into tobacco by Agrobacterium tumefaciens. Molecular identification showed that the ADF4 gene was integrated into the genome of tobacco and expressed in transgenic tobaccos assayed by PCR and RT-PCR (Fig.3). Expression of an Arabidopsis thaliana ADF4 gene in tobacco resulted in morphological change of plants. The effects of ADF4 on transgenic tobaccos growth were as follows: the hypocotyls of transgenic plants were wavy, especially in darkness, whereas those of the control were straight (Fig.4A); the root hairs of transgenic plants were less than the control, and they were also wavy (Fig.4B); the parenchyma cells of transgenic plants were larger than the control and the arrangement of vascular bundle was out of order (Fig.4C); the flowering time of T(2) line was at least 7 days later than the control; the pollen tubes of transgenic plants were shorter than those of the control (Fig.4D).
克隆并测序了拟南芥的肌动蛋白解聚因子4基因（ADF4）（图1、2）。构建了含有ADF4的植物表达载体，并通过根癌农杆菌将其转化到烟草中。分子鉴定表明，ADF4基因已整合到烟草基因组中，并通过PCR和RT-PCR检测在转基因烟草中表达（图3）。拟南芥ADF4基因在烟草中的表达导致了植株形态的变化。ADF4对转基因烟草生长的影响如下：转基因植株的下胚轴呈波浪状，尤其是在黑暗中，而对照植株的下胚轴是直的（图4A）；转基因植株的根毛比对照少，且根毛也是波浪状的（图4B）；转基因植株的薄壁细胞比对照大，维管束排列紊乱（图4C）；T(2)代株系的开花时间比对照至少晚7天；转基因植株的花粉管比对照短（图4D）。
