Xu Jing, Dai Suming, Wang Xue, Gentile Alessandra, Zhang Zhuo, Xie Qingxiang, Su Yajun, Li Dazhi, Wang Bing
College of Plant Protection, Hunan Agricultural University, Changsha 410128, China.
National Citrus Improvement Center, Hunan Agricultural University, Changsha 410128, China.
Plants (Basel). 2023 Aug 25;12(17):3054. doi: 10.3390/plants12173054.
The actin-depolymerizing factor (ADF) gene family regulates changes in actin. However, the entire ADF family in the sweet orange has not been systematically identified, and their expressions in different organs and biotic stress have not been determined. In this study, through phylogenetic analysis of the sweet orange ADF gene family, seven CsADFs were found to be highly conserved and sparsely distributed across the four chromosomes. Analysis of the cis-regulatory elements in the promoter region showed that the gene had the potential to impact the development of sweet oranges under biotic or abiotic stress. Quantitative fluorescence analysis was then performed. Seven were differentially expressed against the invasion of and Las pathogens. It is worth noting that the expression of was significantly up-regulated at 4 days post-infection. Subcellular localization results showed that CsADF4 was localized in both the nucleus and the cytoplasm. Overexpression of enhanced the sensitivity of sweet orange leaves to . These results suggest that may regulate the interaction of and bacterial pathogens, providing a way to further explore the function and mechanisms of ADF in the sweet orange.
肌动蛋白解聚因子（ADF）基因家族调控肌动蛋白的变化。然而，甜橙中的整个ADF家族尚未得到系统鉴定，其在不同器官中的表达以及生物胁迫下的表达情况也未确定。在本研究中，通过对甜橙ADF基因家族进行系统发育分析，发现7个CsADF基因高度保守且分散分布在4条染色体上。对启动子区域顺式调控元件的分析表明，这些基因有可能在生物或非生物胁迫下影响甜橙的发育。随后进行了定量荧光分析。7个基因在面对柑橘黄龙病菌和韧皮部杆菌病原体入侵时差异表达。值得注意的是，CsADF4在感染后4天表达显著上调。亚细胞定位结果表明，CsADF4定位于细胞核和细胞质中。CsADF4的过表达增强了甜橙叶片对柑橘黄龙病菌的敏感性。这些结果表明，CsADF4可能调控甜橙与细菌病原体的相互作用，为进一步探究ADF在甜橙中的功能和机制提供了途径。
