Maurya Dharmendra Kumar, Nair Cherupally Krishnan Krishnan
Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, 400 085, Maharashtra, India.
Mol Cell Biochem. 2006 Apr;285(1-2):181-90. doi: 10.1007/s11010-005-9079-1. Epub 2006 Feb 14.
Our previous study showed that ferulic acid (FA) offered good radioprotection under in vitro and in vivo conditions to DNA and enhanced the DNA repair process in the peripheral blood leucocytes of mice in vivo. This study concerns radioprotection of normal versus tumor cells. Administration of FA (50 mg/kg body weight) to mice bearing fibrosarcoma tumor, 1 h prior to/ or immediately after radiation exposure (4 Gy) showed preferential radioprotection to normal cells i.e. peripheral blood leucocytes and bone marrow cells in comparison to tumor cells. This preferential protection under in vivo conditions could be attributed to poor vasculature in the tumor or peculiar characteristics of the tumor cells either to restrict its entry inside the cells or metabolize or inactivate the drug. To resolve these ex vivo study was carried out using bone marrow and tumor cells. It was found that under ex vivo condition also only bone marrow cells were protected by FA. Thus the studies revealed that FA showed preferential protection to normal cells under both in vivo and ex vivo conditions.
我们之前的研究表明，阿魏酸（FA）在体外和体内条件下均能对DNA提供良好的辐射防护，并增强小鼠体内外周血白细胞的DNA修复过程。本研究关注正常细胞与肿瘤细胞的辐射防护。在携带纤维肉瘤肿瘤的小鼠接受辐射暴露（4 Gy）前1小时或暴露后立即给予FA（50 mg/kg体重），结果显示与肿瘤细胞相比，FA对正常细胞（即外周血白细胞和骨髓细胞）具有优先辐射防护作用。体内条件下的这种优先保护作用可能归因于肿瘤血管系统不佳，或者肿瘤细胞具有特殊特性，要么限制FA进入细胞内，要么使其代谢或失活。为了解决这些问题，使用骨髓细胞和肿瘤细胞进行了体外研究。结果发现，在体外条件下，也只有骨髓细胞受到FA的保护。因此，这些研究表明，FA在体内和体外条件下均对正常细胞具有优先保护作用。
