Amala Cancer Research Centre, Thrissur 680555, Kerala, India.
Pushpagiri Institute of Medical Sciences & Research Centre, Thiruvalla 689101, Kerala, India.
Environ Toxicol Pharmacol. 2012 Sep;34(2):253-262. doi: 10.1016/j.etap.2012.04.008. Epub 2012 Apr 28.
Tempol (TPL) under in vitro conditions reduced the extent of gamma radiation induced membrane lipid peroxidation and disappearance of covalently closed circular form of plasmid pBR322. TPL protected cellular DNA from radiation-induced damage in various tissues under ex vivo and in vivo conditions as evidenced by comet assay. TPL also prevented radiation induced micronuclei formation (in peripheral blood leucocytes) and chromosomal aberrations (in bone marrow cells) in whole body irradiated mice. TPL enhanced the rate of repair of cellular DNA (blood leucocytes and bone marrow cells) damage when administered immediately after radiation exposure as revealed from the increased Cellular DNA Repair Index (CRI). The studies thus provided compelling evidence to reveal the effectiveness of TPL to protect hematopoietic system from radiation injury.
在体外条件下，Tempol（TPL）减少了γ辐射诱导的膜脂质过氧化和质粒 pBR322 共价闭合环状形式的消失。TPL 通过彗星试验证明，在体外和体内条件下，可防止各种组织中细胞 DNA 受到辐射损伤。TPL 还可防止全身照射小鼠外周血白细胞中的辐射诱导微核形成和骨髓细胞中的染色体畸变。TPL 通过增加细胞 DNA 修复指数（CRI），当在辐射暴露后立即给药时，增强了细胞 DNA（白细胞和骨髓细胞）损伤的修复速度。因此，这些研究提供了令人信服的证据，表明 TPL 可有效保护造血系统免受辐射损伤。
