Sato Katsuhiko, Anzai Jun-Ichi
Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai, 980-8578, Japan.
Anal Bioanal Chem. 2006 Mar;384(6):1297-301. doi: 10.1007/s00216-005-0279-z. Epub 2006 Feb 14.
The fluorescence of fluoresceinisothiocyanate-labeled concanavalin A (FITC-Con A) was quenched by forming an FITC-Con A-glycogen conjugate and dequenched upon addition of sugars to the conjugate solution due to disaggregation of the conjugate. However, fluorescence quenching was barely observed upon formation of FITC-Con A-dextran conjugate. The sugar-induced fluorescence response of the FITC-Con A-glycogen conjugate depended significantly on the type of sugar: methylated alpha-D: -glucose and alpha-D: -mannose both induced high and rapid responses, while the responses to D: -mannose and D: -glucose were moderate. In contrast, no response was observed in the presence of D: -galactose due to a lack of affinity to Con A. Thus, it is apparent that D: -glucose and other sugars can be detected via the fluorescence of the FITC-Con A-glycogen conjugate.
异硫氰酸荧光素标记的伴刀豆球蛋白A（FITC-Con A）的荧光通过形成FITC-Con A-糖原共轭物而猝灭，并且由于共轭物的解聚，在向共轭物溶液中添加糖后荧光恢复。然而，在形成FITC-Con A-葡聚糖共轭物时几乎观察不到荧光猝灭。FITC-Con A-糖原共轭物的糖诱导荧光响应显著取决于糖的类型：甲基化α-D-葡萄糖和α-D-甘露糖均诱导高且快速的响应，而对D-甘露糖和D-葡萄糖的响应适中。相比之下，由于对Con A缺乏亲和力，在D-半乳糖存在下未观察到响应。因此，很明显D-葡萄糖和其他糖可以通过FITC-Con A-糖原共轭物的荧光进行检测。
