Chemical Resources Laboratory, Tokyo Institute of Technology, 4259-R1-13 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan.
Chem Asian J. 2010 Apr 1;5(4):817-24. doi: 10.1002/asia.200900430.
The synthesis of mannose-substituted tetraphenylethenes (TPEs) and their aggregation-induced emission (AIE) behavior, induced by interactions with concanavalin A (Con A), are reported. A mixture of the mannose-TPE conjugates and Con A in a buffer solution displays an intense blue emission on agglutination within a few seconds, which serves as a "turn-on" fluorescent sensor for lectins. The sensing is also selective: the conjugates act as a sensor for Con A, but do not sense a galactose-binding lectin, PNA. Con A-recognition is not affected even in the presence of other proteins in a mixture. The conjugates also exhibit high sensitivity to detect Con A. An increased sensitivity of the conjugates results if mannopyranoside substituents are linked to the TPE-core unit with a flexible chain and/or when the number of mannose residues increases.
报道了甘露糖取代的四苯乙烯（TPE）的合成及其与伴刀豆球蛋白 A（Con A）相互作用诱导的聚集诱导发光（AIE）行为。在缓冲溶液中，甘露糖-TPE 缀合物和 Con A 的混合物在几秒钟内发生凝集，会发出强烈的蓝色发射，可作为凝集素的“开启”荧光传感器。这种感应具有选择性：缀合物可作为 Con A 的传感器，但不能感应半乳糖结合凝集素 PNA。即使在混合物中存在其他蛋白质，Con A 的识别也不受影响。这些缀合物对 Con A 的检测也具有很高的灵敏度。如果甘露吡喃糖苷取代基与 TPE-核心单元通过柔性链连接，或者当甘露糖残基数增加时，缀合物的灵敏度会更高。
