Baricević I, Jones D R, Nikolić J A, Nedić O
Institute for the Application of Nuclear Energy-INEP, Banatska 31b, Belgrade, Serbia and Montenegro.
Horm Metab Res. 2006 Jan;38(1):22-7. doi: 10.1055/s-2006-924972.
Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important anabolic functions in normal tissue growth, which in excess may lead to tumorigenesis. In the present study, circulating IGF-I, IGF-II and their binding proteins (IGFBP-3, IGFBP-2 and IGFBP-1) were determined in 92 adult patients with gastrointestinal inflammation (Crohn's disease, colitis ulcerosa, gastritis, duodenitis errosiva, gastrointestinal candidiasis, and rotaviral and adenoviral enteritis). Serum IGF concentrations were measured by radioimmunoassay, while IGFBP profiles and IGFBP proteolytic patterns were characterized by immunoblotting. Concentrations of both IGF-I and IGF-II were significantly (p < 0.001) lower in patients than in healthy subjects. Immunoblotting demonstrated a decreased amount of intact IGFBP-3 (by approximately 60%), whereas IGFBP-2 and IGFBP-1 were increased (approximately 1.7 and 3.5-fold, respectively). No alteration in either fragmentation pattern or relative degree of proteolysis was detected in patients compared to the control group. It may be concluded that the IGF system is seriously imbalanced in patients with gastrointestinal inflammation, regardless of primary cause. These findings may help towards a better understanding of the metabolic outcome of the inflammatory process, and possibly in predicting the efficiency of patient recovery.
胰岛素样生长因子（IGFs）及其结合蛋白（IGFBPs）在正常组织生长中具有重要的合成代谢功能，其过量可能导致肿瘤发生。在本研究中，测定了92例患有胃肠道炎症（克罗恩病、溃疡性结肠炎、胃炎、糜烂性十二指肠炎、胃肠道念珠菌病以及轮状病毒和腺病毒肠炎）的成年患者的循环IGF-I、IGF-II及其结合蛋白（IGFBP-3、IGFBP-2和IGFBP-1）。通过放射免疫测定法测量血清IGF浓度，同时通过免疫印迹法对IGFBP谱和IGFBP蛋白水解模式进行表征。患者体内IGF-I和IGF-II的浓度均显著低于健康受试者（p < 0.001）。免疫印迹显示完整的IGFBP-3量减少（约60%），而IGFBP-2和IGFBP-1增加（分别约为1.7倍和3.5倍）。与对照组相比，未检测到患者在片段化模式或蛋白水解相对程度上有任何改变。可以得出结论，无论原发性病因如何，胃肠道炎症患者的IGF系统严重失衡。这些发现可能有助于更好地理解炎症过程的代谢结果，并可能用于预测患者康复的效果。
