Zhang Kai, Choi Heyok, Dionysiou Dionysios D, Sorial George A, Oerther Daniel B
Civil and Environmental Engineering, University of Cincinnati, Cincinnati, OH, USA.
Environ Microbiol. 2006 Mar;8(3):433-40. doi: 10.1111/j.1462-2920.2005.00909.x.
More effective control of membrane biofouling in membrane bioreactors (MBRs) lies in the fundamental understanding of the pioneer microorganisms responsible for surface colonization that leads to biofilm formation. In this study, the composition of the planktonic and sessile microbial communities inhabiting four laboratory-scale MBR systems were compared using amplified ribosomal DNA restriction analysis (ARDRA) and 16S ribosomal DNA gene sequencing. The ARDRA results suggest that the microbial communities on membrane surfaces could be very different from the ones in the suspended biomass. Phylogenetic analysis based on the 16S rRNA gene sequences provided a list of bacteria that might be the pioneers of surface colonization on microfiltration membranes. The results further suggested that research on the mechanisms of cell attachment in such an engineering environment could be critical for future development of appropriate biofouling control strategies.
更有效地控制膜生物反应器（MBR）中的膜生物污染，关键在于深入了解那些导致生物膜形成的表面定植的先锋微生物。在本研究中，使用扩增核糖体DNA限制性分析（ARDRA）和16S核糖体DNA基因测序，比较了四个实验室规模的MBR系统中浮游和固着微生物群落的组成。ARDRA结果表明，膜表面的微生物群落可能与悬浮生物质中的微生物群落有很大不同。基于16S rRNA基因序列的系统发育分析列出了可能是微滤膜表面定植先锋的细菌名单。结果进一步表明，在这种工程环境中对细胞附着机制的研究对于未来制定合适的生物污染控制策略可能至关重要。
