在大肠杆菌中，OmpC和OmpF是在pH 8以上的高渗胁迫下生长所必需的。

OmpC and OmpF are required for growth under hyperosmotic stress above pH 8 in Escherichia coli.

作者信息

Kaeriyama M, Machida K, Kitakaze A, Wang H, Lao Q, Fukamachi T, Saito H, Kobayashi H

机构信息

Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, Japan.

出版信息

Lett Appl Microbiol. 2006 Mar;42(3):195-201. doi: 10.1111/j.1472-765X.2006.01845.x.

DOI:10.1111/j.1472-765X.2006.01845.x

PMID:16478504

Abstract

AIMS

To investigate the requirement of outer membrane porins for osmotic adaptation at alkaline pH in Escherichia coli.

METHODS AND RESULTS

Escherichia coli mutants deficient in ompC, ompF and both genes were constructed and the growth of these mutants was observed at alkaline pH. The growth rate of the mutant deficient in both ompC and ompF was slower than that of the wild type and mutants deficient in one of these genes under hyperosmotic stress at pHs above 8.0. The decreased rate was recovered when a cloned ompC was introduced to the mutant, but the growth recovery with a cloned ompF was partial. Such growth diminution was not observed at pHs below 8.0.

CONCLUSION

OmpC and OmpF were shown to participate in hyperosmotic adaptation at alkaline pH in E. coli.

SIGNIFICANCE AND IMPACT OF THE STUDY

This study is the first report to demonstrate that OmpC and OmpF are required for hyperosmotic adaptation at pHs above 8.0, but not below 8.0.

摘要

目的

研究大肠杆菌外膜孔蛋白在碱性pH值下对渗透适应的需求。

方法与结果

构建了ompC、ompF及二者均缺失的大肠杆菌突变体，并观察这些突变体在碱性pH值下的生长情况。在pH值高于8.0的高渗胁迫下，ompC和ompF均缺失的突变体的生长速率比野生型及仅缺失其中一个基因的突变体慢。当将克隆的ompC导入该突变体时，生长速率下降的情况得以恢复，但导入克隆的ompF时生长恢复不完全。在pH值低于8.0时未观察到这种生长减缓现象。