Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260–8675, Japan.
Biol Pharm Bull. 2011;34(3):330-4. doi: 10.1248/bpb.34.330.
The roles of OmpC and OmpF in acidic resistance (AR) were examined. When ompC and ompF were deleted, AR was decreased. The decreased level of AR seen in the mutant that was deficient in ompC and ompF was elevated by the addition of glutamate, but not by the addition of arginine or lysine. The expression levels of adiA and cadB were diminished by the deletion of ompC and ompF, and the conversion of arginine to agmatine and lysine to cadaverine by intact cells were reduced in the mutant. The expression of gadA/gadB was not affected by the deletion of ompC and ompF. These results suggest that the transport of arginine, lysine, and their decarboxylated products through OmpC and/or OmpF is essential for the survival of Escherichia coli cells under extremely acidic conditions.
研究了 OmpC 和 OmpF 在酸性抗性 (AR) 中的作用。当 ompC 和 ompF 缺失时，AR 降低。缺失 ompC 和 ompF 的突变体中 AR 的降低水平通过添加谷氨酸而升高，但通过添加精氨酸或赖氨酸则不会升高。adiA 和 cadB 的表达水平因 ompC 和 ompF 的缺失而降低，完整细胞中精氨酸向胍丁胺和赖氨酸向尸胺的转化减少。gadA/gadB 的表达不受 ompC 和 ompF 缺失的影响。这些结果表明，精氨酸、赖氨酸及其脱羧产物通过 OmpC 和/或 OmpF 的转运对于大肠杆菌细胞在极其酸性条件下的存活是必不可少的。
