Meile Jean-Christophe, Wu Ling Juan, Ehrlich S Dusko, Errington Jeff, Noirot Philippe
Laboratoire de Génétique Microbienne UR895, INRA-Domaine de Vilvert, Jouy en Josas, France.
Proteomics. 2006 Apr;6(7):2135-46. doi: 10.1002/pmic.200500512.
Construction and microscopic imaging of protein fusions to green fluorescent protein (GFP) have revolutionised our understanding of bacterial structure and function. We have undertaken a systematic study of the localisation of over 100 Bacillus subtilis proteins, following the development of high-throughput construction and analysis procedures. We focused on proteins linked in various ways to the DNA replication machinery, as well as on proteins exemplifying a range of other cellular functions and structures. The results validate the approach as a way of obtaining systematic protein localisation information. They also provide a range of novel biological insights, particularly through the identification of a number of proteins not previously known to be associated with the DNA replication factory.
与绿色荧光蛋白（GFP）融合的蛋白质构建及显微成像，彻底改变了我们对细菌结构和功能的理解。在开发出高通量构建和分析程序后，我们对100多种枯草芽孢杆菌蛋白质的定位进行了系统研究。我们聚焦于以各种方式与DNA复制机制相关联的蛋白质，以及代表一系列其他细胞功能和结构的蛋白质。这些结果证实了该方法是获取系统蛋白质定位信息的一种方式。它们还提供了一系列新颖的生物学见解，特别是通过鉴定出许多以前未知与DNA复制工厂相关的蛋白质。
