Gille Christian, Spring Baerbel, Tewes Lena, Poets Christian F, Orlikowsky Thorsten
University Children's Hospital, Department of Neonatology, 72076 Tübingen, Germany.
Cytometry A. 2006 Mar;69(3):152-4. doi: 10.1002/cyto.a.20222.
Interactions between innate and adaptive immune functions in neonatal macrophages (MPhi) are still unclear. We therefore established a method to quantify bacterial phagocytosis and intracellular degradation, using green fluorescent protein (GFP)-labeled Escherichia coli in combination with phenotypic analysis. The kinetics of the proportion of phagocyting MPhi, phagocytosed bacteria per MPhi, and bacterial degradation were comparable for cord blood MPhi of term neonates and MPhi of healthy adults. Phenotyping revealed CD14 and CD16 to be down-modulated within minutes. GFP-labeled E. coli may be useful tools to further study MPhi subpopulations and determinants of phagocytosis in cord blood MPhi.
新生儿巨噬细胞（MPhi）中固有免疫和适应性免疫功能之间的相互作用仍不清楚。因此，我们建立了一种方法，将绿色荧光蛋白（GFP）标记的大肠杆菌与表型分析相结合，以量化细菌吞噬作用和细胞内降解。足月新生儿脐带血MPhi和健康成年人的MPhi在吞噬MPhi的比例、每个MPhi吞噬的细菌数量以及细菌降解的动力学方面具有可比性。表型分析显示，CD14和CD16在数分钟内被下调。GFP标记的大肠杆菌可能是进一步研究脐带血MPhi中MPhi亚群和吞噬作用决定因素的有用工具。
