Arroyo C M, Kohno M
Veterans Administration Medical Center, Baltimore, Maryland 21218.
Free Radic Res Commun. 1991;14(2):145-55. doi: 10.3109/10715769109094127.
The spin trapping technique was used in an attempt to detect the free radical nitric oxide (NO) in solution. Five different spin traps were examined, alpha-phenyl-N-tert butyl nitrone (PBN), alpha-(4-pyridyl-N-oxide) N-tert-butylnitrone (POBN), 5,5-dimethyl-pyrroline-N-oxide (DMPO), 2-methyl-2-nitrosopropane (MNP) and 3,5-dibromo-4-nitrosobenzene sulfonate (DBNBS). Our results suggest that the nitroso spin traps (MNP, DBNBS) are better suited for the identification of NO-related signals, than the nitrones, DMPO, PBN and POBN. In addition, it is shown that spin trapping of NO-related signals with nitroso and nitrone spin traps is subject to many artifacts.
采用自旋捕集技术试图检测溶液中的自由基一氧化氮（NO）。研究了五种不同的自旋捕集剂，α-苯基-N-叔丁基硝酮（PBN）、α-（4-吡啶-N-氧化物）N-叔丁基硝酮（POBN）、5,5-二甲基-吡咯啉-N-氧化物（DMPO）、2-甲基-2-亚硝基丙烷（MNP）和3,5-二溴-4-亚硝基苯磺酸盐（DBNBS）。我们的结果表明，亚硝基自旋捕集剂（MNP、DBNBS）比硝酮类捕集剂DMPO、PBN和POBN更适合用于识别与NO相关的信号。此外，研究表明，用亚硝基和硝酮自旋捕集剂对与NO相关的信号进行自旋捕集会产生许多假象。
