Hohwy Thomas, Otkjaer Kristian, Madsen Jens, Søerensen Gritt, Nielsen Ole, Vestergaard Christian, Steiniche Torben, Holmskov Uffe, Lomholt Hans
Department of Dermatology and Venerology, Aarhus University Hospital, P.P. Ørumsgade 11, DK-8000 Aarhus C, Denmark.
Exp Dermatol. 2006 Mar;15(3):168-74. doi: 10.1111/j.1600-0625.2006.00406.x.
The collectin surfactant protein-D (SP-D) shows antimicrobial and immuno-regulatory properties and has recently been detected in the basal layers of normal human skin. This molecule potentially plays an important role in inflammatory skin diseases and therefore SP-D content and location was examined using immunohistochemistry on skin biopsies from patients with the two major dermatologic diseases, psoriasis and atopic dermatitis. SP-D was located in the stratum basale of all biopsies with similar intense staining in both diseased and normal skin. Differences were detected in stratum spinosum where involved psoriatic skin showed intense staining through the entire region significantly different from uninvolved and normal skin. Lesional atopic skin showed moderate staining extending through the basal three-fourths of stratum spinosum. Using real time polymerase chain reaction analysis, no substantial up-regulation of SP-D mRNA was detected in lesional psoriatic skin, and a comparison of serum levels of SP-D between patients with atopic dermatitis or psoriasis and a group of age matched healthy controls did not show significant differences. In conclusion SP-D was significantly more abundant in the stratum spinosum of lesional psoriatic and atopic skin due to more cells producing the molecule rather than up-regulation of production in single cells of diseased skin. Further studies are needed to show if SP-D plays a role in the protection against skin infections or modulation of the inflammatory process in these common skin diseases.
凝集素表面活性蛋白-D（SP-D）具有抗菌和免疫调节特性，最近在正常人皮肤的基底层被检测到。该分子可能在炎症性皮肤病中发挥重要作用，因此，利用免疫组织化学方法，对患有两种主要皮肤病——银屑病和特应性皮炎的患者的皮肤活检样本进行检测，以研究SP-D的含量和定位。在所有活检样本的基底层均检测到SP-D，在患病皮肤和正常皮肤中的染色强度相似。在棘层检测到差异，受累的银屑病皮肤在整个区域均显示强染色，与未受累皮肤和正常皮肤有显著差异。特应性病变皮肤在棘层底部的四分之三区域显示中度染色。利用实时聚合酶链反应分析，在银屑病病变皮肤中未检测到SP-D mRNA的大量上调，比较特应性皮炎或银屑病患者与一组年龄匹配的健康对照者的血清SP-D水平，未发现显著差异。总之，在银屑病和特应性病变皮肤的棘层中，SP-D明显更为丰富，这是由于产生该分子的细胞增多，而非患病皮肤单个细胞中产量上调所致。需要进一步研究以确定SP-D在预防这些常见皮肤病的皮肤感染或调节炎症过程中是否发挥作用。
