Hanson Charles, Caisander Gunilla
Department of Obstetrics and Gynecology, Göteborg University, Sahlgrenska University Hospital, Göteborg, Sweden.
APMIS. 2005 Nov-Dec;113(11-12):751-5. doi: 10.1111/j.1600-0463.2005.apm_305.x.
The use of human embryonic stem cells (HESC) in research is increasing exponentially and HESC will certainly be of importance in biological, clinical and toxicological research for many years to come. Once established, HESC lines are expected to be chromosomally stable. However, our own experience of culturing HESC and some published reports indicate that HESC may show chromosomal instability while being cultured continuously in vitro. We conclude that the effects of different culture techniques and long-term culture on the chromosome stability of HESC still remain to be elucidated and we recommend regular analysis of the chromosome constitution in cell lines using traditional karyotyping, CGH, FISH and PCR. We also recommend freezing of HESC at low passage number and in larger batches after thawing and expansion in order to secure material in case mutations occur in the cell line at a later stage of culture.
人类胚胎干细胞（HESC）在研究中的应用正呈指数级增长，并且在未来许多年里，HESC在生物学、临床和毒理学研究中肯定会具有重要意义。一旦建立，HESC系预计将具有染色体稳定性。然而，我们自己培养HESC的经验以及一些已发表的报告表明，HESC在体外连续培养时可能会表现出染色体不稳定性。我们得出结论，不同培养技术和长期培养对HESC染色体稳定性的影响仍有待阐明，我们建议使用传统核型分析、比较基因组杂交（CGH）、荧光原位杂交（FISH）和聚合酶链反应（PCR）对细胞系的染色体组成进行定期分析。我们还建议在低传代次数时对HESC进行冷冻，并在解冻和扩增后以更大批量进行冷冻，以便在培养后期细胞系发生突变时确保有可用材料。
