Wang Zhan-ke, Xu Lin-shui, Wang Shi-liang, Liu Long-yan, Hu Xin-yong, Zhu Zhong-zhen, Zhang Yu-zhen, Xiong Xiao-ping, Yu Wen
Department of Clinical Laboratory, The 94th Hospital of PLA, Nanchang 330002, P. R. China.
Zhonghua Shao Shang Za Zhi. 2005 Dec;21(6):422-5.
To evaluate the influence of glucose-insulin-potassium treatment (GIK) on the levels of inflammatory cytokines in the scalded rats with MODS.
One hundred and twenty Sprague Dawley rats were inflicted with 30% TBSA full-thickness scalding, and MODS model was reproduced with intraperitoneal injection of endotoxin following burn injury. Then the rats were randomly divided into GIK, glucose (G) and control (C) groups, with 40 rats in each group. The serum contents of glucose, lactate acid, TNF-alpha, NO and IL-6 of the rats in the three groups were determined during 1 to 7 PSD, and the mortality rate within 7 PSD was observed.
The serum contents of glucose, lactate acid, TNF-alpha, NO and IL-6 of the rats in the GIK group were obviously lower than those in the other two groups during 1 to 7 PSD (P < 0.01), and reached the lowest level at 6 to 7 PSD (TNF-alpha: 2.37 +/- 0.54 microg/L; IL-6: 0.28 +/- 0.17 microg/L; NO: 29 +/- 9 micromol/L). The content of glucose and lactate acid in G group were obviously higher than those in control group (P < 0.01), but the contents of TNF-alpha, IL-6 and NO content were similar between these two groups (P > 0.05). The mortality in GIK group within 7 PSD was 20.0%, which was evidently lower than that in G (37.5%) and C (47.5%) groups (P < 0.05), while that between G and C groups was similar (P > 0.05).
The administration of GIK might ameliorate sepsis by reducing the levels of inflammatory cytokine after burns and endotoxin challenge.
评估葡萄糖 - 胰岛素 - 钾治疗（GIK）对多器官功能障碍综合征（MODS）烫伤大鼠炎症细胞因子水平的影响。
将120只Sprague Dawley大鼠造成30% 体表面积的全层烫伤，伤后腹腔注射内毒素复制MODS模型。然后将大鼠随机分为GIK组、葡萄糖（G）组和对照组（C），每组40只。在伤后1至7天观察期内测定三组大鼠血清葡萄糖、乳酸、肿瘤坏死因子 -α（TNF -α）、一氧化氮（NO）和白细胞介素 -6（IL -6）的含量，并观察伤后7天内的死亡率。
在伤后1至7天观察期内，GIK组大鼠血清葡萄糖、乳酸、TNF -α、NO和IL -6的含量明显低于其他两组（P < 0.01），并在伤后6至7天达到最低水平（TNF -α：2.37 ± 0.54微克/升；IL -6：0.28 ± 0.17微克/升；NO：29 ± 9微摩尔/升）。G组葡萄糖和乳酸含量明显高于对照组（P < 0.01），但两组TNF -α、IL -6和NO含量相似（P > 0.05）。GIK组伤后7天内死亡率为20.0%，明显低于G组（37.5%）和C组（47.5%）（P < 0.05），而G组和C组之间死亡率相似（P > 0.05）。
GIK治疗可能通过降低烧伤和内毒素攻击后炎症细胞因子水平来改善脓毒症。
