State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Sci China Life Sci. 2013 May;56(5):453-9. doi: 10.1007/s11427-013-4472-6. Epub 2013 May 1.
Coxiella burnetii is the etiological agent of Q fever. To identify its major seroreactive proteins, a subgenomic protein array was developed. A total of 101 assumed virulence-associated recombinant proteins of C. burnetii were probed with sera from mice experimentally infected with C. burnetii and sera from Q fever patients. Sixteen proteins were recognized as major seroreactive antigens by the mouse sera. Seven of these 16 proteins reacted positively with at least 45% of Q fever patient sera. Notably, HspB had the highest fluorescence intensity value and positive frequency of all the proteins on the array when probed with both Q fever patient sera and mouse sera. These results suggest that these seven major seroreactive proteins, particularly HspB, are potential serodiagnostic and subunit vaccine antigens of Q fever.
贝氏考克斯体是 Q 热的病原体。为了鉴定其主要的血清反应蛋白，开发了一个亚基因组蛋白阵列。用感染贝氏考克斯体的实验小鼠血清和 Q 热患者血清探测了 101 种假设的与毒力相关的重组考克斯体蛋白。16 种蛋白被小鼠血清识别为主要的血清反应抗原。这 16 种蛋白中有 7 种与至少 45%的 Q 热患者血清呈阳性反应。值得注意的是，在与 Q 热患者血清和小鼠血清探测时，HspB 在阵列上的所有蛋白中具有最高的荧光强度值和阳性频率。这些结果表明，这七种主要的血清反应蛋白，特别是 HspB，是 Q 热潜在的血清学诊断和亚单位疫苗抗原。
