果蝇细胞系中单个基因Gart、Notch和white中紫外线诱导的嘧啶二聚体的修复。

Repair of UV-induced pyrimidine dimers in the individual genes Gart, Notch and white from Drosophila melanogaster cell lines.

作者信息

de Cock J G, Klink E C, Ferro W, Lohman P H, Eeken J C

机构信息

MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.

出版信息

Nucleic Acids Res. 1991 Jun 25;19(12):3289-94. doi: 10.1093/nar/19.12.3289.

DOI:10.1093/nar/19.12.3289

PMID:1648203

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328324/

Abstract

The excision repair of UV-induced pyrimidine dimers was investigated in three genes: Gart, Notch and white in a permanent Drosophila cell line Kc, derived from wild type Drosophila melanogaster embryonic cells. In this cell line Gart and Notch are actively transcribed, whereas white is not expressed. In all three genes UV-induced pyrimidine dimers were removed with the same rate and to the same extent: 60% removal within 16 hours, up to 80-100% in 24 hours after irradiation with 10 or 15 J/m2 UV. These kinetics are similar to the time course of dimer removal measured in the genome overall. No difference in repair of the inactive white locus compared to the active Gart and Notch genes was found. Similar results were obtained using a different wild type cell line, SL2, although repair appeared to be somewhat slower in this cell line. The results are discussed with respect to the data found for gene specific repair in other eukaryotic systems.

摘要

在源自野生型黑腹果蝇胚胎细胞的永久性果蝇细胞系Kc中，对紫外线诱导的嘧啶二聚体的切除修复进行了研究，涉及三个基因：Gart、Notch和white。在该细胞系中，Gart和Notch被积极转录，而white不表达。在所有这三个基因中，紫外线诱导的嘧啶二聚体以相同的速率和相同的程度被去除：在16小时内去除60%，在用10或15 J/m2紫外线照射后24小时内去除率高达80 - 100%。这些动力学与在整个基因组中测量的二聚体去除时间进程相似。与活跃的Gart和Notch基因相比，未发现无活性的white基因座在修复上有差异。使用不同的野生型细胞系SL2也获得了类似的结果，尽管在该细胞系中修复似乎稍慢一些。结合在其他真核系统中发现的基因特异性修复数据对结果进行了讨论。