de Cock J G, van Hoffen A, Wijnands J, Molenaar G, Lohman P H, Eeken J C
MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.
Nucleic Acids Res. 1992 Sep 25;20(18):4789-93. doi: 10.1093/nar/20.18.4789.
The nucleotide excision repair (NER; dark-repair) of (6-4)photoproducts ((6-4)PPs) was assayed in cells from a permanent Drosophila melanogaster embryonic cell line, Kc, after exposure to 20 or 40 J/m2 ultraviolet (UV) light. Induction rates in the transcriptionally active genes Gart and Notch as well as in the inactive white locus is similar. They are formed with a frequency of about one-third of that of cyclobutane pyrimidine dimers (CPDs). In all three genes, (6-4)PPs are repaired with the same rate and to the same extent: 31% of the (6-4)PPs are removed in 4 hours post-irradiation and after 16 hours repair is nearly complete. In none of the three genes strand-specific repair was found. Exposure of cells that were irradiated with 40 J/m2 UV to photoreactivating light for 1 hour prior to dark-repair incubation, resulted in enhanced repair of (6-4)PPs.
在将永久性黑腹果蝇胚胎细胞系Kc的细胞暴露于20或40 J/m²紫外线（UV）后，检测了（6-4）光产物（（6-4）PPs）的核苷酸切除修复（NER；暗修复）情况。转录活性基因Gart和Notch以及无活性的白眼基因座中的诱导率相似。它们的形成频率约为环丁烷嘧啶二聚体（CPDs）的三分之一。在所有这三个基因中，（6-4）PPs以相同的速率和程度进行修复：31%的（6-4）PPs在照射后4小时被去除，16小时后修复几乎完成。在这三个基因中均未发现链特异性修复。在暗修复孵育前，将用40 J/m²紫外线照射的细胞暴露于光复活光下1小时，导致（6-4）PPs的修复增强。
