黑腹果蝇Kc细胞中活性基因中环丁烷嘧啶二聚体的链特异性修复缺失。

Absence of strand-specific repair of cyclobutane pyrimidine dimers in active genes in Drosophila melanogaster Kc cells.

作者信息

de Cock J G, Klink E C, Lohman P H, Eeken J C

机构信息

MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.

出版信息

Mutat Res. 1992 Aug;274(2):85-92. doi: 10.1016/0921-8777(92)90055-8.

DOI:10.1016/0921-8777(92)90055-8

PMID:1378210

Abstract

Strand-specific excision repair of UV-induced cyclobutane pyrimidine dimers was investigated in three genes: Gart, Notch and white in the permanent Kc cell line derived from wild-type Drosophila melanogaster embryonic cells. In this cell line Gart and Notch are transcriptionally active, whereas white is not expressed. Cells were irradiated with 10 or 15 J/m2 ultraviolet (UV) light (predominantly 254 nm). In all three genes, cyclobutane pyrimidine dimers were removed from the non-transcribed strand at the same rate and to the same extent as from the transcribed strand, indicating the absence of strand-specific repair in permanent Drosophila embryonic cell lines.

摘要

在源自野生型黑腹果蝇胚胎细胞的永久性Kc细胞系中，对紫外线诱导的环丁烷嘧啶二聚体的链特异性切除修复进行了研究，涉及三个基因：Gart、Notch和white。在该细胞系中，Gart和Notch具有转录活性，而white不表达。用10或15 J/m2的紫外线（主要为254 nm）照射细胞。在所有这三个基因中，环丁烷嘧啶二聚体从非转录链上的去除速率和程度与从转录链上相同，这表明永久性果蝇胚胎细胞系中不存在链特异性修复。