Shiono H, Maseda C, Akane A, Matsubara K
Department of Legal Medicine, Shimane Medical University, Izumo, Japan.
Am J Forensic Med Pathol. 1991 Mar;12(1):50-3. doi: 10.1097/00000433-199103000-00009.
We developed a head-space method for the determination of blood cyanide by gas chromatography with electron-capture detection. In this technique, a reaction precolumn packed with chloramine-T was used for the conversion of hydrogen cyanide into cyanogen chloride. Since the reaction precolumn eliminated the necessity of trapping hydrogen cyanide from biological samples, blood cyanide could be analyzed quickly by acidification only. Using this method, blood cyanide levels of fire victims were determined at autopsy. The serum values of cyanide ranged from 0.11 micrograms/ml to 18.12 micrograms/ml. However, a significantly higher cyanide content was detected in the left ventricular blood than in the right. This indicates that death was caused by the fire and suggests that the collecting point of the blood sample is an important factor in the determination of inhaled cyanide. There was a positive correlation between blood cyanide and carboxyhemoglobin contents.
我们开发了一种顶空法，通过带有电子捕获检测的气相色谱法测定血液中的氰化物。在该技术中，填充有氯胺 - T的反应预柱用于将氰化氢转化为氯化氰。由于反应预柱消除了从生物样品中捕集氰化氢的必要性，仅通过酸化就可以快速分析血液中的氰化物。使用这种方法，在尸检时测定了火灾受害者的血液氰化物水平。氰化物的血清值范围为0.11微克/毫升至18.12微克/毫升。然而，在左心室血液中检测到的氰化物含量明显高于右心室。这表明死亡是由火灾引起的，并表明血液样本的采集点是测定吸入氰化物的一个重要因素。血液氰化物与碳氧血红蛋白含量之间存在正相关。
