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[Site-directed mutagenesis with the use of addressed fragmentation of single-stranded DNA for obtaining hybrids of homologous proteins].

作者信息

Shekhter I I, Ratmanova K I, Veĭko V P, Debabov V G

出版信息

Bioorg Khim. 1991 Mar;17(3):379-86.

PMID:1648356
Abstract

Site-directed multiple mutagenesis to obtain hybrids of homologous proteins was carried out by means of the oligonucleotide-targeting digestion of ss DNA. The procedure is more convenient, rapid and simple than the step-by-step approach. To demonstrate different approaches to targeting digestion of ss DNA, NcoI, HinfI, FokI endonucleases were used for DNA cleavage. A hydride of the human and porcine IFN-alpha-2-genes has been constructed.

摘要

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